ISO-SENSITEST BROTH

Code: CM0473

a semi defined medium designed for antimicrobial susceptibility testing

Typical Formula*	gm/litre
Hydrolysed casein	11.0
Peptones	3.0
Glucose	2.0
Sodium chloride	3.0
Soluble starch	1.0
Disodium hydrogen phosphate	2.0
Sodium acetate	1.0
Magnesium glycerophosphate	0.2
Calcium gluconate	0.1
Cobaltous sulphate	0.001
Cupric sulphate	0.001
Zinc sulphate	0.001
Ferrous sulphate	0.001
Manganous chloride	0.002
Menadione	0.001
Cyanocobalamin	0.001
L-Cysteine hydrochloride	0.02
L-Tryptophan	0.02
Pyridoxine	0.003
Pantothenate	0.003
Nicotinamide	0.003
Biotin	0.0003
Thiamine	0.00004
Adenine	0.01
Guanine	0.01
Xanthine	0.01
Uracil	0.01
pH 7.4 ± 0.2  @ 25°C

*Adjusted as required to meet performance standards

Directions
Add 23.4g to 1 litre of distilled water. Mix well and heat to dissolve. Distribute into tubes or bottles. Sterilise by autoclaving at 121°C for 15 minutes.

Description
Oxoid Iso-Sensitest™ Broth has been produced in parallel with Iso-Sensitest Agar CM0471. The broth has an identical nutrient formulation without the specially purified agar. Where studies on antimicrobial susceptibilities are to be made in both broth and agar, this will be found to be of particular value. Details of the function of the medium and the methodology used for antimicrobial susceptibility tests are discussed in the Section, Susceptibility Testing.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Pale straw coloured solution