英文名称： Calibrated ELISA Standards
Recombinant cytokine and chemokine standards calibrated for ELISA.
Thermo Scientific Pierce Calibrated ELISA Standards are designed for use in sandwich ELISAs. Each standard provides material for a minimum of one standard curve run in duplicate or triplicate and has been calibrated to an international reference standard (NIBSC or NIAID reference lots). Calibrated ELISA Standards can be used to generate standard curves in ELISA assays or used to calibrate or "value assign" bulk recombinant material.
Calibrating bulk recombinant proteins
Many suppliers offer recombinant cytokines and chemokines for use in cell culture and other applications. In most cases, these reagents are supplied in quantities of 5 to 100µg per vial and sometimes include a packaging overfill. (A 20µg vial may actually contain 22µg.) Package claim is based on mass, not on immunoreactivity as measured in ELISA. In order to utilize a bulk recombinant for ELISA, it must be "value assigned". An assay is run using the Calibrated ELISA Standard to make the standard curve. The bulk recombinant protein is then diluted in the range of the standard curve and each dilution is read as an "unknown." The calculated concentration vs. the expected concentration will determine the value assignment of the starting bulk recombinant material.
Example procedure for value assignment of bulk recombinant proteins
If the starting material (bulk recombinant) is a 5µg vial and it is reconstituted with 500µL of diluent, then diluted 1:10 in a buffer containing carrier protein, the concentration is assumed to be 10µg/mL. If the assay range for the ELISA in development is 15 to 1,000pg/mL, the bulk recombinant would be diluted to 2,000pg/mL, 1,000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, and so on. Each dilution is treated as an unknown sample and evaluated in the ELISA based on the Calibrated ELISA Standard curve. The calculated concentration in ELISA vs. the expected concentration based on the label claim of the bulk recombinant will determine the value assignment. For example, the assumed dilution of 250pg/mL may actually read 400pg/mL in the ELISA (40% higher). In this case, the starting material assumed to be 250ng/mL would be "value assigned" at 400ng/mL. The dilution that falls on the most linear portion of the curve should be used to determine the value assignment of the bulk recombinant material. Value-assigned recombinant should be frozen in aliquots to avoid repeated freeze-thaw cycles.
We offer a full line of Calibrated ELISA Standards for Inflammatory and Th1/Th2 cytokines. The lot-specific Certificate of Analysis for each Standard indicates the international reference standard used for calibration.
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Cytokine and Chemokine ELISA Kits
ELISA Screening Sets
ELISA Mini Kits
Recombinant Proteins
Calibrated to NIBSC reference lot 88/646
Calibrated to NIAID reference lot Gxg-01-902-535
Calibrated to NIBSC reference lot 86/632
Calibrated to NIBSC reference lot 86/680
Calibrated to NIBSC reference lot 86/504
Calibrated to NIBSC reference lot 88/656
Calibrated to NIBSC reference lot 90/586
Calibrated to NIBSC reference lot 89/514
Calibrated to NIBSC reference lot 89/520
Calibrated to NIBSC reference lot 93/722
Calibrated to NIBSC reference lot 95/544
Calibrated to NIBSC reference lot 94/622
Calibrated to Thermo Scientific Internal Reference Standard
Calibrated to NIAID reference lot 87/650
Calibrated to NIBSC reference lot Gg-02-901-533
Calibrated to Thermo Scientific Internal Reference Standard
Calibrated to Thermo Scientific Internal Reference Standard
Calibrated to NCI-BRB reference lot 91/656
Calibrated to Thermo Scientific Internal Reference Standard
Calibrated to NIBSC reference lot 93/730
Calibrated to Thermo Scientific Internal Reference Standard
Calibrated to Thermo Scientific Internal Reference Standard
Calibrated to NIBSC reference lot 88/532