Stabilized HRP Conjugates are secondary antibodies and biotin-binding protein conjugates with horseradish peroxidase (HRP) enzyme that are stabilized in pre-diluted form for greater accuracy and convenience in preparing probing solutions for ELISA, Western blotting and other detection procedures. The conjugates are accurately prepared, dispensed and supplied at 10 µg/ml (100X more dilute than typical 1 mg/ml preparations), eliminating the inaccuracies associated with two-stage dilution schemes required with traditional conjugate preparations for use with chemiluminescent substrates and other high-sensitivity detection methods. The liquid formulation of each prediluted HRP-conjugate is stable at 4°C, eliminating the need to freeze stock solutions for storage.

Our Goat Anti-Mouse IgG Whole Molecule (GAM), Goat Anti-Rabbit IgG Whole Molecule (GAR), streptavidin and NeutrAvidin Proteins are conjugated to HRP using optimized manufacturing procedures that ensure complete conjugation of the protein to HRP. This is a key feature, as any unconjugated protein would compete with the conjugated material and cause lower signal intensity in downstream applications. We have used our HRP conjugates in ELISA, Western blotting and immunohistochemistry (IHC) applications, demonstrating their competence in these applications.

Superb Western blot results with Thermo Scientific Pierce Stabilized HRP Conjugates. Western blot of HeLa lysate samples probed with a mouse anti-GAPDH antibody (1:2,000 dilution of 1 mg/ml stock solution) and then Stabilized Goat Anti-Mouse-HRP (4 ng/ml, 1:2,500 dilution of 10µg/ml stock solution). HRP activity was detected with SuperSignal West Femto Chemiluminescent Substrate (Product #34095). If a typical 1 mg/ml stock solution of secondary antibody had been used, it would have required a 250,000-fold dilution, which is both tedious, prone to pipetting error and usually wasteful.

Highlights

• Obtain high signal-to-noise ratio – ideal for Western Blotting, ELISA and IHC applications
• Get reproducible results – avoid inconsistencies caused by lot-to-lot variation and magnification of pipetting error during dilution
• Save time – stabilized liquid formats eliminate the need to freeze aliquots; no waiting time for thawing aliquots
• Ready to use – format of the stabilized liquid conjugates provides ease of use and handling

Related Products
High-Sensitivity HRP Conjugates
Poly-HRP Conjugates
Traditional Secondary Antibody Conjugates
Other Streptavidin, NeutrAvidin and Avidin Conjugates

Product #	Description	Pkg. Size
21134	High Sensitivity Streptavidin-HRP (10 µg/ml)	2 ml
32430	Stabilized Goat Anti-Mouse IgG (H+L), Peroxidase Conjugated (10 µg/ml)	2 ml
32460	Stabilized Goat Anti-Rabbit IgG (H+L), Peroxidase Conjugated (10 µg/ml)	2 ml