人TNFsRIElisa试剂盒 英文名称: Human TNFsRI ELISA Kit | |
型号:null 产品货号: EK0528 | |
价格:请致电:010-57128832,18610462672 | |
品牌: 美国 | |
产品:人TNFsRIElisa试剂盒 货号:EK0528 Description TNFsRI (also known as soluble tumor necrosisFactor receptor 1) is a cytokine receptor that binds tumor necrosisFactors (TNF). Chan et al. (2000)Found that in contrast to the p60 (TNFRSF1A) and p80 (TNFRSF1B; 191191), in the absence of a ligand, TNFA receptors self-assemble through a distinctFunctional domain in the TNFR extracellular domain, termed the pre-ligand assembly domain (PLAD). Deletion of PLAD results in monomeric presentation of p60 or p80.Flow cytometric analysis showed that efficient TNFA binding depends on receptor self-assembly. They alsoFound that other members of the TNF receptor superfamily, including the extracellular domains of TRAIL (TNFRSF10A; 603611), CD40 (109535), andFAS (TNFRSF6; 134637), all self-associate, but do not interact with heterologous receptors. By Southern blot analysis of human/Chinese hamster somatic cell hybrid DNA, Milatovich et al. (1991) mapped the TNFR1 gene to 12pter-cen. Derre et al. (1991)Found by non-radioactive in situ hybridization that the type 1 receptor (the p55 TNF receptor) is encoded by a gene located on chromosome 12p13.2. ScienCells human TNFsRI ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human TNFsRI-specific monoclonal antibodies (clone No. 28401.111) are pre-coated onto 8 x 12 strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human TNFa in the sample that is captured on the strips.
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.
Shipped on gel ice
1. Chan,F. K.-M., Chun, H. J., Zheng, L., Siegel, R. M., Bui, K. L., Lenardo, M. J. A domain in TNF receptors that mediates ligand-independent receptor assembly and signaling. Science 288: 2351-2354, 2000.
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