Description

Tumor necrosisFactor a (TNFa or TNF) is secreted by macrophages in response to inflammation, infection, and cancer. Rat Tumor NecrosisFactor (TNF) and Lymphotoxin (TNFβ) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNFa is produced by monocytes, which can stimulate endothelial cells to produce the multi-lineage growthFactor granulocyte-macrophage colony-stimulatingFactor (GM-CSF) and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells, but has no effect on normal cells. Rat TNF has been purified to apparent homogeneity as a 17.3kDa proteinFrom HL-60 leukemia cells and has shown cytotoxic and cytostatic activities against various rat tumor cell lines. The Rat TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNFa has been mapped to human chromosome 6. The standard product used in this kit is recombinant rat TNFa, consisting of 157 amino acids with a molecular mass of 17kDa.

ScienCells rat TNFa ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat TNFa-specific monoclonal antibodies are pre-coated onto 8 x 12 strips. The rat-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of rat TNFa in the sample that is captured on the strips.

Product Use

This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.

Storage

Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.

Shipping

Shipped on gel ice

Reference

1. Brenner, D. A.; OHara, M.; Angel, P.; Chojkier, M.; Karin, M. Prolonged activation of JUN and collagenase genes by tumour necrosisFactora. Nature 337: 661-663, 1989.
2. Nedwin, G. E.; Naylor, S. L.; Sakaguchi, A. Y.; Smith, D.; Jarrett-Nedwin, J.; Pennica, D.; Goeddel, D. V.; Gray, P. W. Human lymphotoxin and tumor necrosisFactor genes: structure, homology and chromosomal localization. Nucleic Acids Res. 13: 6361-6373, 1985.
3. Broudy, V. C.; Kaushansky, K.; Segal, G. M.; Harlan, J. M.; Adamson, J. W. Tumor necrosisFactor type a stimulates human endothelial cells to produce granulocyte/macrophage colony-stimulatingFactor. Proc. Nat. Acad. Sci. 83: 7467-7471, 1986.
4. Wang, A. M.; Creasey, A. A.; Ladner, M. B.; Lin, L. S.; Strickler, J.; Van Arsdell, J. N.; Yamamoto, R.; Mark, D.F. Molecular cloning of the complementary DNAFor human tumor necrosisFactor. Science 228: 149-154, 1985.