Description

In theField of cell biology, TNF-related apoptosis-inducing ligand (TRAIL) is a protein thatFunctions as a ligand to induce the programmed process of cell death, called apoptosis. TRAIL has also been designated CD253 (cluster of differentiation 253). Tumor necrosisFactor (TNF)Family cytokinesFunction as prominent mediators of immune regulation and inflammatory response. Most TNFFamily cytokines are expressed as type II transmembrane proteins, with homology confined to approximately 150 C-terminal residues. The TNF ligands interact with a parallelFamily of receptors. TRAIL binds to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII). The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. TRAIL also binds the receptors DcR1 and DcR2, which either do not contain a cytoplasmic domain (DcR1) or contain a truncated death domain (DcR2). The standard product used in this kit is recombinant human TRAIL with a molecular mass of 21 kDa. It is expressedFrom the amino acids T95-G28.

ScienCells human TRAIL ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human TRAIL-specific polyclonal antibodies are pre-coated onto 8 x 12 strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human TRAIL in the sample that is captured on the strips.

Product Use

This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.

Storage

Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.

Shipping

Shipped on gel ice

Reference

1. Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl JK, Sutherland GR, Smith TD, Rauch C, Smith CA (December 1995). "Identification and characterization of a new member of the TNFFamily that induces apoptosis". Immunity 3 (6): 673?82.
2. Pitti RM, Marsters SA, Ruppert S, Donahue CJ, Moore A, Ashkenazi A (May 1996). "Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosisFactor cytokineFamily". J. Biol. Chem. 271 (22): 12687?90.
3. Song JJ, Lee YJ (May 2008). "Differential cleavage of Mst1 by caspase-7/-3 is responsibleFor TRAIL-induced activation of the MAPK superfamily". Cell. Signal. 20 (5): 892?906.