Description

Vascular permeability Factor/vascular endothelial growthFactor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis, and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 andFlt-1. The VEGF gene is mapped byFluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant rat VEGF164, which is a 25 kDa single chain, as well as a 50 kDa dimer.

ScienCells rat VEGF ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat VEGF-specific monoclonal antibodies are pre-coated onto 8 x 12 divisible strips. The rat-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of rat VEGF in the sample that is captured on the strips.

Product Use

This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.

Storage

Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.

Shipping

Shipped on gel ice

Reference

1. Basu, S.; Nagy, J. A.; Pal, S.; Vasile, E.; Eckelhoefer, I. A.; Bliss, V. S.; Manseau, E. J.; Dasgupta, P. S.; Dvorak, H. F.; Mukhopadhyay, D. The neurotransmitter dopamine inhibits angiogenesis induced by vascular permeability Factor/vascular endothelial growthFactor. Nature Med. 7: 569-574, 2001.
2. Poulaki, V.; Mitsiades, C. S.; McMullan, C.; Sykoutri, D.;Fanourakis, G.; Kotoula, V.; Tseleni-Balafouta, S.; Koutras, D. A.; Mitsiades, N. Regulation of vascular endothelial growthFactor expression by insulin-like growthFactor I in thyroid carcinomas. J. Clin. Endocr. Metab. 88: 5392-5398, 2003.
3. Awata, T.; Inoue, K.; Kurihara, S.; Ohkubo, T.; Watanabe, M.; Inukai, K.; Inoue, I.; Katayama, S. A common polymorphism in the 5-prime-untranslated region of the VEGF gene is associated with diabetic retinopathy in type 2 diabetes. Diabetes 51: 1635-1639, 2002.
4. Soker, S.; Takashima, S.; Miao, H. Q.; Neufeld, G.; Klagsbrun, M. Neuropilin-1 is expressed by endothelial and tumor cells as an isoform-specific receptorFor vascular endothelial growthFactor. Cell 92: 735-745, 1998.
5. Wei, M.-H.; Popescu, N. C.; Lerman, M. I.; Merrill, M. J.; Zimonjic, D. B. Localization of the human vascular endothelial growthFactor gene, VEGF, at chromosome 6p12. Hum. Genet. 97: 794-797, 1996.