| 小鼠血管细胞粘附分子-1ELISA试剂盒 英文名称： Mouse VCAM-1 ELISA Kit | |
| 型号:null 产品货号： EK0538 | |
| 价格:请致电：010-57128832,18610462672 | |
| 品牌: 美国 | |
产品：小鼠血管细胞粘附分子-1ELISA试剂盒 货号：EK0538 Description Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesiveFor certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Increased expression of VCAM-1 is associated with a variety of chronic inflammatory conditions, making its expression andFunction a targetFor therapeutic intervention. Integrin α4β1 (VLA-4) and VCAM-1Facilitate a critical cell-cell adhesion event requiredFor survival of endothelial and mural cells during vascularization. The VCAM-1 gene is located on the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with a molecular mass of 101.9 kDa. As a result of glycosylation, SDS-PAGE reveals a molecular mass of 120kDa. ScienCells mouse VCAM-1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse VCAM-1-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The mouse-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of mouse VCAM-1 in the sample that is captured on the strips. Product Use This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. Storage Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles. Shipping Shipped on gel ice Reference 1. Cybulsky, M. I.;Fries, J. W. U.; Williams, A. J.; Sultan, P.; Eddy, R.; Byers, M.; Shows, T.; Gimbrone, M. A., Jr.; Collins, T. Gene structure, chromosomal location, and basisFor alternative mRNA splicing of the human VCAM1 gene. Proc. Nat. Acad. Sci. 88: 7859-7863, 1991. 2. Besemer, J.; Harant, H.; Wang, S.; Oberhauser, B.; Marquardt, K.;Foster, C. A.; Schreiner, E. P.; de Vries, J. E.; Dascher-Nadel, C.; Lindley, I. J. D. Selective inhibition of cotranslational translocation of vascular cell adhesion molecule 1. (Letter) Nature 436: 290-293, 2005. 3. Garmy-Susini, B.; Jin, H.; Zhu, Y.; Sung, R.-J.; Hwang, R.; Varner, J. Integrin alpha-4-beta-1--VCAM-1--mediated adhesion between endothelial and mural cells is requiredFor blood vessel maturation. J. Clin. Invest. 115: 1542-1551, 2005.
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