| 人胎球蛋白AELISA试剂盒 英文名称： HumanFetuin-A ELISA Kit | |
| 型号:null 产品货号： EK0757 | |
| 价格:请致电：010-57128832,18610462672 | |
| 品牌: 美国 | |
产品：人胎球蛋白AELISA试剂盒 货号：EK0757 Description Alpha2-HS glycoprotein (AHSG), also known asFetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification. Human plasma protein AHSG is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter (Lee et al., 1987). Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization. ScienCells humanFetuin-A ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. HumanFetuin-A-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of humanFetuin-A in the sample that is captured on the strips. Product Use This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. Storage Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles. Shipping Shipped on gel ice Reference 1. Osawa, M., Tian, W., Horiuchi, H., Kaneko, M., Umetsu, K. Association of alpha-2-HS glycoprotein (AHSG,Fetuin-A) polymorphism with AHSG and phosphate serum levels. Hum. Genet. 116: 146-151, 2005. 2. Magnuson, V. L., McCombs, J. L., Lee, C.-C., Yang,F., Bowman, B. H., McGill, J. R. Human alpha-2-HS-glycoprotein localized to 3q27-q29 by in situ hybridization. Cytogenet. Cell Genet. 47: 72-74, 1988. 如需要其他产品资料请发邮件至wwwfudan@163.com索取
|