| 人血管内皮生长因子ELISA试剂盒 英文名称： Human VEGF ELISA Kit | |
| 型号:null 产品货号： EK0539 | |
| 价格:请致电：010-57128832,18610462672 | |
| 品牌: 美国 | |
产品：人血管内皮生长因子ELISA试剂盒 货号：EK0539 Description Vascular endothelial growthFactor (VEGF) is expressed in multiple cells and tissues including skeletal and cardiac muscle, hepatocytes, osteoblasts, neutrophils, macrophages, keratinocytes, brown adipose tissue, CD34+ stem cells, endothelial cells,Fibroblasts, and vascular smooth muscle cells. VEGF expression is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-a. VEGF is expressed by most malignant tumors, has critical roles in vasculogenesis, and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis, and plays a key role in the pathophysiology of several neoplasias. In diseased tissues, VEGF promotes vascular permeability and contributes to tumor metastasis by promoting both extravasation and tumor angiogenesis. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF binds to two receptor tyrosine kinases, KDR/Flk-1 andFlt-1. The VEGF gene is mapped byFluorescence in situ hybridization to chromosome 6p12. ScienCells human VEGF ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human VEGF specific monoclonal capture antibodies are pre-coated onto 8 x 12 strips. The human-specific polyclonal detection antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added next and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human VEGF in the sample that is captured on the strips. Product Use This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. Storage Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles. Shipping Shipped on gel ice Reference 1. Basu, S.; Nagy, J. A.; Pal, S.; Vasile, E.; Eckelhoefer, I. A.; Bliss, V. S.; Manseau, E. J.; Dasgupta, P. S.; Dvorak, H. F.; Mukhopadhyay, D. The neurotransmitter dopamine inhibits angiogenesis induced by vascular permeability Factor/vascular endothelial growthFactor. Nature Med. 7: 569-574, 2001. 2. Poulaki, V.; Mitsiades, C. S.; McMullan, C.; Sykoutri, D.;Fanourakis, G.; Kotoula, V.; Tseleni-Balafouta, S.; Koutras, D. A.; Mitsiades, N. Regulation of vascular endothelial growthFactor expression by insulin-like growthFactor I in thyroid carcinomas. J. Clin. Endocr. Metab. 88: 5392-5398, 2003. 3. Awata, T.; Inoue, K.; Kurihara, S.; Ohkubo, T.; Watanabe, M.; Inukai, K.; Inoue, I.; Katayama, S. A common polymorphism in the 5-prime-untranslated region of the VEGF gene is associated with diabetic retinopathy in type 2 diabetes. Diabetes 51: 1635-1639, 2002. 4. Soker, S.; Takashima, S.; Miao, H. Q.; Neufeld, G.; Klagsbrun, M. Neuropilin-1 is expressed by endothelial and tumor cells as an isoform-specific receptorFor vascular endothelial growthFactor. Cell 92: 735-745, 1998. 5. Wei, M.-H.; Popescu, N. C.; Lerman, M. I.; Merrill, M. J.; Zimonjic, D. B. Localization of the human vascular endothelial growthFactor gene, VEGF, at chromosome 6p12. Hum. Genet. 97: 794-797, 1996.
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