17-0618-01 PROTEIN G SEPHAROSE 4 FF, 5 ML

17-0618-02 PROTEIN G SEPHAROSE 4 FF, 25 M

17-0618-05 PROTEIN G SEPHAROSE 4FF 200 ML

17-0640-01 ACT THIOL SEPHAROSE 4B 15 G

17-0690-01 LYSINE-SEPHAROSE 4B 15 G

17-0700-01 2,5 ADP-SEPHAROSE 4B 5 G

17-0756-01 GLUTATHIONE SEPHAROSE 4B 10 ML

17-0756-04 GLUTATHIONE SEPHAROSE 4B, 300ML

17-0756-05 GLUTATHIONE SEPHAROSE 4B, 100M

17-0780-01 PROTEIN A-SEPHAROSE CL-4B 1.5G

17-0820-01 CNBR ACTIVATED SEPHAROSE 6MB

17-0885-01 GAMMABIND G SEPHAROSE, 5 ML

17-0885-02 GAMMABIND G SEPHAROSE, 25 ML

17-0885-04 GAMMABIND G SEPHAROSE, 250 ML

17-0886-01 GAMMABIND PLUS SEPHAROSE, 5 ML

17-0886-02 GAMMABIND PLUS SEPHAROSE, 25 M

17-0886-04 GAMMABIND PLUS SEPHAROSE, 500ML

17-0906-01 NHS ACT SEPHAROSE 4 FF 25 ML

17-0920-06 IMAC SEPHAROSE HP, 25 ML

17-0920-07 IMAC SEPHAROSE HP, 100 ML

17-0921-07 IMAC SEPHAROSE 6 FF, 25 ML

17-0921-08 IMAC SEPHAROSE 6 FF, 100 ML

17-0948-01 BLUE SEPHAROSE 6 FAST FLOW, 50ML

17-0956-01 GELATIN-SEPHAROSE 4B, 25 ML

17-0963-02 PROTEIN A SEPHAROSE CL-4B 500M

17-0963-03 PROTEIN A SEPHAROSE CL-4B, 25

17-0969-01 IGG SEPHAROSE 6 FAST FLOW 10

Protein G Sepharose™ 4 Fast Flow

High capacity, high flow rate fractionation of monoclonal antibodies at both laboratory and process scales, without binding albumin.
Rapid processing of large volumes, recoveries of 70-90%.
Binds to all IgG subclasses from human, mouse, and rat; binds total IgG from guinea pig, rabbit, goat, cow, sheep, and horse

Protein G Sepharose™ 4 Fast Flow

Technical Information

TECHNICAL SPECIFICATIONS

Ligand Recombinant protein G lacking albumin-binding region

Ligand coupling method Cyanogen bromide activation

No.of IgG binding 2 sites per ligand

MW of ligand ~17 000

pI of ligand 4.1

Degree of substitution ~2 mg Protein G/ml drained medium

Number of IgG binding sites per ligand 2

Matrix Highly cross-linked agarose, 4%

Binding capacity > 20 mg human IgG/ml medium

Average particle size 90 μm

Ligand density » 2 mg Protein G/ml medium

Chemical stability Stable to all commonly used aqueous buffers - 1 M acetic acid, 1% SDS, and 6 M guanidine HCl (tested at 37°C for 7 days), as well as, 0.1 M glycine NaOH pH 11, 1 M HC1, and 8 M urea (stable for at least 2 h at room temperature)

pH stability 3-9 (working), 2*-10 (short term)

Physical stability Negligible volume variation due to changes in pH or ionic strength

Sanitization Do not autoclave.Wash the column with 70% ethanol.

Antimicrobial agent 20% ethanol

Storage 20% ethanol

Storage temperature 4°C to 8°C

* pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Purification of human monoclonal antibody using Protein G Sepharose™ 4 Fast Flow (1).

Reference

Jungbauer, A. et al. Comparison of protein A, protein G and copolymerised hydroxyapatite for the purification of human monoclonal antibodies. J. Chromatogr. 476, 257 (1989).