| 小鼠Gp130/IL6STELISA试剂盒 英文名称： Mouse Gp130/IL6ST ELISA Kit | |
| 型号:null 产品货号： EK0577 | |
| 价格:请致电：010-57128832,18610462672 | |
| 品牌: 美国 | |
产品：小鼠Gp130/IL6STELISA试剂盒 货号：EK0577 Description Gp130 (Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein that is theFounding member of the class of all cytokine receptors. The Gp130 gene is mapped to 5q11.2. Gp130 shows an apparent molecular mass of 130 kDa by SDS-PAGE. Gp130 is involved in theFormation of high-affinity IL6-binding sites and in IL6 signal transduction. Soluble Gp130 has a role in modulating signals transduced by membrane-bound Gp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments cannot transduce a growth signal. ScienCells mouse Gp130 ELISA Kit ias based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse Gp130-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The mouse-specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of mouse Gp130 in the sample that is captured on the strips. Product Use This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. Storage Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles. Shipping Shipped on gel ice Reference 1. Hibi, M., Murakami, M., Saito, M., Hirano, T., Taga, T., Kishimoto, T. Molecular cloning and expression of an IL-6 signal transducer, gp130. Cell 63: 1149-1157, 1990. 2. Narazaki, M., Yasukawa, K., Saito, T., Ohsugi, Y.,Fukui, H., Koishihara, Y., Yancopoulos, G. D., Taga, T., Kishimoto, T. SolubleForms of the interleukin-6 signal-transducing receptor component gp130 in human serum possessing a potential to inhibit signals through membrane-anchored gp130. Blood 82: 1120-1126, 1993. 3. Yoshida K, Taga T, Saito M, Suematsu S, Kumanogoh A, Tanaka T,Fujiwara H, Hirata M, Yamagami T, Nakahata T, Hirabayashi T, Yoneda Y, Tanaka K, Wang W-Z, Mori C, Shiota K, Yoshida N, Kishimoto T (1996).
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