| 大鼠IL-1αELISA试剂盒 英文名称： Rat IL-1α ELISA Kit | |
| 型号:null 产品货号： EK0390 | |
| 价格:请致电：010-57128832,18610462672 | |
| 品牌: 美国 | |
产品：大鼠IL-1αELISA试剂盒 货号：EK0390 Description Interleukin-1a (IL-1a) and interleukin-1β (IL-1β) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1a has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1a is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1a gene is assigned to chromosome 2. Genetic polymorphisms at IL-1a and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1a and IL-1β genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant rat IL-1a with the molecular mass of 18kDa. ScienCells rat IL-1a ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat IL-1a specific-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The rat specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of rat IL-1a captured in strips. Product Use This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. Storage Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles. Shipping Shipped on gel ice. Reference 1. Lafage, M.; Maroc, N.; Dubreuil, P.; de Waal Malefijt, R.; Pebusque, M.-J.; Carcassonne, Y.; Mannoni, P. The human interleukin-1-alpha gene is located on the long arm of chromosome 2 at band q13. Blood 73: 104-107, 1989. 2. Bailly, S.; di Giovine, F. S.; Blakemore, A. I. F.; Duff, G. W. Genetic polymorphism of human interleukin-1-alpha. Europ. J. Immun. 23: 1240-1245, 1993. 3. Modi, W. S.; Masuda, A.; Yamada, M.; Oppenheim, J. J.; Matsushima, K.; OBrien, S. J. Chromosomal localization of the human interleukin 1a (IL-1-alpha) gene. Genomics 2: 310-314, 1988. 4. Diehl, S. R.; Wang, Y.; Brooks, C. N.; Burmeister, J. A.; Califano, J. V.; Wang, S.; Schenkein, H. A. Linkage disequilibrium of interleukin-1 genetic polymorphisms with early-onset periodontitis. J. Periodont. 70: 418-430, 1999. 5. Silver, A. R. J.; Masson, W. K.; George, A. M.; Adam, J.; Cox, R. The Il-1 alpha and beta genes are closely linked (less than 70 kb) on mouse chromosome 2. Somat. Cell Molec. Genet. 16: 549-556, 1990.
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