| 大鼠IL-4ELISA试剂盒 英文名称： Rat IL-4 ELISA Kit | |
| 型号:null 产品货号： EK0406 | |
| 价格:请致电：010-57128832,18610462672 | |
| 品牌: 美国 | |
产品：大鼠IL-4ELISA试剂盒 货号：EK0406 Description Interleukin-4 (IL-4), also known as B-cell stimulatory factor1 (BSF1), is an immuno-modulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immuno-surveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. Rat IL-4 is a 13.5 KDa glycoprotein, consisting of 147 amino acids in precursor form and 123 amino acids in mature form. ScienCells rat IL-4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat IL-4-specific monoclonal antibodies are precoated onto 8 x 12 strips. The rat specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of rat IL-4 in the sample that is captured in the strips. Product Use This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. Storage Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles. Shipping Shipped on gel ice. Reference 1. Prokopchuk, O.; Liu, Y.; Henne-Bruns, D.; Kornmann, M. Interleukin-4 enhances proliferation of human pancreatic cancer cells: evidence for autocrine and paracrine actions. Br J Cancer.2005 Mar 14;92(5):921-8. 2. Arai, N.; Nomura, D.; Villaret, D.; DeWaal Malefijt, R.; Seiki, M.; Yoshida, M.; Minoshima, S.; Fukuyama, R.; Maekawa, M.; Kudoh, J.; Shimizu, N.; Yokota, K.; Abe, E.; Yokota, T.; Takebe, Y.; Arai, K. Complete nucleotide sequence of the chromosomal gene for human IL-4 and its expression. J. Immun. 142: 274-282, 1989. 如需要其他产品资料请发邮件至wwwfudan@163.com索取
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