| 人TNFβELISA试剂盒 英文名称： Human TNFβ ELISA Kit | |
| 型号:null 产品货号： EK0584 | |
| 价格:请致电：010-57128832,18610462672 | |
| 品牌: 美国 | |
产品：人TNFβELISA试剂盒 货号：EK0584 Description Tumor necrosisFactor β (TNFβ), previously called lymphotoxin-alpha (LTA), is a cytokine produced by lymphocytes. TNFa and TNFβ share 35% identity and 50% homology in amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immuno-stimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with a molecular mass of 18.8 kDa. ScienCells human TNF? ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human TNFβ-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human TNF? in the sample that is captured on the strips. Product Use This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. Storage Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles. Shipping Shipped on gel ice Reference 1. Nakayama, T.; Soma M.; Sato, N.; Haketa, A.; Kosuge, K.; Aoi, N.; Sato, M.; Izumi, Y.; Matsumoto, K.; Kanmatsuse, K.; Kokubun, S. An association study in essential hypertension usingFunctional polymorphisms in lymphotoxin-alpha gene. Am J Hypertens. 2004 Nov;17(11 Pt 1):1045-9. 2. Aggarwal, B. B.; Eessalu, T. E.; Hass, P. E. Characterization of receptorsFor human tumour necrosisFactor and their regulation by gamma-interferon. Nature 318: 665-667, 1985. 3. Knight, J. C.; Keating, B. J.; Kwiatkowski, D. P. Allele-specific repression of lymphotoxin-alpha by activated B cellFactor-1. Nature Genet. 36: 394-399, 2004. 4. Nedwin, GE.; Jarrett-Nedwin, J.; Smith, DH.; Naylor, SL.; Sakaguchi, AY.; Goeddel, DV.; Gray, PW. Structure and chromosomal localization of the human lymphotoxin gene. J Cell Biochem. 1985;29(3):171-81.
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