| 人APP ELISA试剂盒 英文名称： Human APP ELISA Kit | |
| 型号:null 产品货号： EK0658 | |
| 价格:请致电：010-57128832,18610462672 | |
| 品牌: 美国 | |
产品：人APP ELISA试剂盒 货号：EK0658 Description Amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and is concentrated in the synapses of neurons. Its primaryFunction is not known, though it has been implicated as a regulator of synapseFormation, neural plasticity and iron export. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates β-amyloid (Aβ), a 39- to 42-amino acid peptide whose amyloidFibrillarForm is the primary component of amyloid plaquesFound in the brains of Alzheimers disease patients. APP undergoes post-translational proteolytic processing by a-, β-, and γ-secretases. a-secretase generates soluble amyloid protein, while β- and γ-secretases generate APP components with amyloidogenicFeatures. These 2 processing pathways are mutually exclusive. ScienCells human APP ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human APP-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human APP in the sample that is captured on the strips. Product Use This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. Storage Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles. Shipping Shipped on gel ice Reference 1. Wang Y, Ha Y (August 2004). "The X-ray structure of an antiparallel dimer of the human amyloid precursor protein E2 domain". Mol. Cell 15 (3): 343?53. 2. Priller C, Bauer T, Mitteregger G, Krebs B, Kretzschmar HA, Herms J (July 2006). "SynapseFormation andFunction is modulated by the amyloid precursor protein". J. Neurosci. 26 (27): 7212?21. 3. Turner PR, OConnor K, Tate WP, Abraham WC (May 2003). "Roles of amyloid precursor protein and itsFragments in regulating neural activity, plasticity and memory". Prog. Neurobiol. 70 (1): 1?32. 4. Duce JA et al. (2010). "Iron-ExportFerroxidase Activity of ?- Amyloid Precursor Protein Is Inhibited by Zinc in Alzheimers Disease". Cell 142 (6): 857?67. 如需要其他产品资料请发邮件至wwwfudan@163.com索取
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