Description

Transforming growthFactor-β1 (TGF-β1) is a multifunctional peptide that controls proliferation, differentiation, and otherFunctions in many cell types. Many cells synthesize TGF-β and essentially all of them have specific receptorsFor this peptide. TGF-β regulates the actions of many other peptide growthFactors and can have a positive or negative regulatory affect.1 TGF-β1 is knownFor its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGF-β1 is also an important mediator of bone remodeling. TGF-β1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growthFactors at the site of inflammation. TGF-β1, a multifunctional cytokine withFibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-β1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGF-β1 is secreted as a latentForm, which consists of its matureForm and a latency-associated peptide (β1-LAP) in either the presence or the absence of additional latent TGF-β1-binding protein. The standard product used in this kit is recombinant TGF-β1 with a molecular mass of 25kDa.

ScienCells rat TGF-β1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat TGF-β1-specific polyclonal antibodies are pre-coated onto 8 x 12 strips. The rat-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of rat TGF-β1 in the sample that is captured on the strips.

Product Use

This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.

Storage

Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.

Shipping

Shipped on gel ice

Reference

1. Sporn, M. B.; Roberts, A. B.; Wakefield, L. M.; Assoian, R. K. Transforming growthFactorβ: biologicalFunction and chemical structure. Science 233: 532-534, 1986.
2. Blanchette,F.; Day, R.; Dong, W.; Laprise, M.-H.; Dubois, C. M. TGFβ-1 regulates gene expression of its own converting enzymeFurin. J. Clin. Invest. 99: 1974-1983, 1997.
3. Janssens, K.; Gershoni-Baruch, R.; Guanabens, N.; Migone, N.; Ralston, S.; Bonduelle, M.; Lissens, W.; Van Maldergem, L.; Vanhoenacker,F.; Verbruggen, L.; Van Hul, W. Mutations in the gene encoding the latency-associated peptide of TGFβ-1 cause Camurati-Engelmann disease. Nature Genet. 26: 273-275, 2000.
4. Li, A. G.; Wang, D.;Feng, X.-H.; Wang, X.-J. Latent TGF?-1 overexpression in keratinocytes results in a severe psoriasis-like skin disorder. EMBO J. 23: 1770-1781, 2004.
5. Li, B.; Khanna, A.; Sharma, V.; Singh, T.; Suthanthiran, M.; August, P. TGFβ-1 DNA polymorphisms, protein levels, and blood pressure. Hypertension 33: 271-275, 1999.
6. Saito, T.; Kinoshita, A.; Yoshiura, K.; Makita, Y.; Wakui, K.; Honke, K.; Niikawa, N.; Taniguchi, N. Domain-specific mutations of a transforming growthFactor (TGF)β 1 latency-associated peptide cause Camurati-Engelmann disease because of theFormation of a constitutively activeForm of TGFβ 1. J. Biol. Chem. 276: 11469-11472, 2001.