17-0973-05 | PHENYL SEPHAROSE 6FF (HS) 200ML | 17-0973-10 | PHENYL SEPHAROSE 6 FF /HS, 25ML | 17-0978-02 | BUTYL-S SEPHAROSE 6 FF, 200 ML | 17-0978-10 | BUTYL-S SEPHAROSE 6 FF, 25ML | 17-0980-01 | BUTYL SEPHAROSE 4 FAST FLOW 20 | 17-0980-10 | BUTYL SEPHAROSE FAST FLOW, 25 | 17-1014-01 | Q SEPHAROSE HIGH PERF. 75 ML | 17-1082-01 | PHENYL SEPHAROSE HIGH PERF. 75 | 17-1087-01 | SP SEPHAROSE HIGH PERF. 75 ML | 17-1273-01 | SOURCE 30S, 50 ML | 17-1275-01 | SOURCE 30Q, 50 ML | 17-1287-01 | ANX SEPH 4 FF HIGH SUB 500ML | 17-1287-10 | ANX SEPHAROSE FF HIGHSUB 25 ML | 17-5072-01 | Q SEPHAROSE XL, 300 ML | 17-5073-01 | SP SEPHAROSE XL, 300 ML | 17-5316-02 | Capto Q 100 ml | 17-5316-10 | CAPTO Q, 25 ML | 17-5317-02 | Capto MMC 100ml | 17-5317-10 | CAPTO MMC, 25ML | 17-5432-01 | Butyl Sepharose HP, 25 ml | 17-5432-02 | Butyl Sepharose HP, 200ml | 17-5437-01 | Q SEPHAROSE XL VIRUS LIC 300ML | 17-5437-10 | Q SEPHAROSE XL VIRUS LIC 25 ML | 17-5441-01 | CAPTO S, 100 ML | 17-5441-10 | CAPTO S, 25 ML | 17-5443-01 | CAPTO DEAE, 100 ML | 17-5443-10 | CAPTO DEAE, 25 ML | 17-5444-10 | CAPTO adhere 25 ML | Phenyl Sepharose™ 6 Fast Flow - Available with high or low levels of phenyl substitution differing in selectivity, efficiency, and binding capacity.
- The standard (low sub) and high-capacity (high sub) aromatic HIC media are made of highly cross-linked 90-μm agarose beads derivatized with phenyl groups via uncharged, chemically stable ether linkages.
Phenyl Sepharose™ 6 Fast FlowTechnical Information
TECHNICAL SPECIFICATIONS | Ligand density | | Phenyl Sepharose™ 6 Fast Flow (low sub) | 25 μmol/ml medium | Phenyl Sepharose™ 6 Fast Flow (high sub) | 40 μmol/ml medium | Binding capacity | Phenyl Sepharose™ 6 Fast Flow (low sub) | 10 mg IgG/ml medium 24 mg HSA/ml medium | Phenyl Sepharose™ 6 Fast Flow (high sub) | 30 mg IgG/ml medium 36 mg HSA/ml medium | Pressure/flow spec. | 200-400 cm/h, 1 bar XK 50/60 column, bed height 25 cm | pH stability | 2-14 (short term), 3-13 (long term) | Chemical stability | Stable in common buffers, chaotropic agents, detergents, and polar organic solvents. | Average particle size | 90 μm | Storage | 20% ethanol | Storage temperature | 4°C to 30°C | Isolation of a monoclonal antibody, IgG1 with Phenyl Sepharose™ 6 Fast Flow (high sub).
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