重组肠激酶特异性高，纯度高英文名称： recombinant enterokinase
型号:null 产品货号： REK08
价格:请致电：010-57128832,18610462672
品牌: 上海
重组肠激酶特异性高，纯度高 1.产品简介雅心肠激酶是一种高纯度的重组牛肠激酶。该酶经HPLC纯化，纯度高，特异性高，并且不含其他蛋白酶。肠激酶（EC 3.4.21.9）是一个特定的蛋白酶，它可以切割前面含有四个天冬氨酸的赖氨酸羧基端位点：天冬氨酸 - 天冬氨酸 - 天冬氨酸 - 天冬氨酸 - 赖氨酸。肠激酶可去除位于蛋白质N-末端的融合蛋白，以除去不需要的融合标签。 2.产品优势高纯度 (图一) （图二）（图三）高特异性 1）特定的蛋白酶，切割前面含有四个天冬氨酸的赖氨酸羧基端位点：天冬氨酸 - 天冬氨酸 - 天冬氨酸 - 天冬氨酸 - 赖氨酸 (DDDDK) 2）不含其他蛋白酶,无非特异性切割图一：SDS-PAGE检测结果，纯化的肠激酶一个单一的主带图二：1U的EK作用于BUTYL-S SEPHAROSE 6 FF, 25ML
17-0980-01	BUTYL SEPHAROSE 4 FAST FLOW 20
17-0980-10	BUTYL SEPHAROSE FAST FLOW, 25
17-1014-01	Q SEPHAROSE HIGH PERF. 75 ML
17-1082-01	PHENYL SEPHAROSE HIGH PERF. 75
17-1087-01	SP SEPHAROSE HIGH PERF. 75 ML
17-1273-01	SOURCE 30S, 50 ML
17-1275-01	SOURCE 30Q, 50 ML
17-1287-01	ANX SEPH 4 FF HIGH SUB 500ML
17-1287-10	ANX SEPHAROSE FF HIGHSUB 25 ML
17-5072-01	Q SEPHAROSE XL, 300 ML
17-5073-01	SP SEPHAROSE XL, 300 ML
17-5316-02	Capto Q 100 ml
17-5316-10	CAPTO Q, 25 ML
17-5317-02	Capto MMC 100ml
17-5317-10	CAPTO MMC, 25ML
17-5432-01	Butyl Sepharose HP, 25 ml
17-5432-02	Butyl Sepharose HP, 200ml
17-5437-01	Q SEPHAROSE XL VIRUS LIC 300ML
17-5437-10	Q SEPHAROSE XL VIRUS LIC 25 ML

SOURCE™ Media/RESOURCE™ and Tricorn Columns for IEX

SOURCE 15Q and 15S media are ideal for fast, high-resolution purifications and easy scale-up.
Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/ml, and improved stability in organic solvents and pH extremes.
SOURCE 15Q 4.6/100 PE and SOURCE 15S 4.6/100 PE are Tricorn high-performance columns designed for high-resolution purification at lab scale and for optimization studies when scaling up.
RESOURCE columns are prepacked with SOURCE 15Q or SOURCE 15S for fast separation. Separation times using 1 ml RESOURCE columns are less than 3 min at 9.6 ml/min and about 20 min using a peristaltic pump at 1 ml/min.
SOURCE 30Q and 30S media are designed for intermediate purification and large-scale polishing.

SOURCE™ Media/RESOURCE™ and Tricorn Columns for IEX

Technical Information

SOURCE™ Media, RESOURCE™, and Tricorn prepacked ion exchange columns increase productivity.

TECHNICAL SPECIFICATIONS

SOURCE media

Ion exchanger type

SOURCE™ 15Q, 30Q Quaternary ammonium strong anion

SOURCE™ 15S, 30S Methyl sulfonate strong cation

Particle size

SOURCE™ 15Q, 15S 15 μm

SOURCE™ 30Q, 30S 30 μm

Matrix Polystyrene/divinylbenzene

pH stability 2-12 (long term), 1-14 (short term)

Chemical stability Stable in all common ion exchange buffers

Storage 20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (S)

Storage temperature 4°C to 30°C

RESOURCE™ prepacked columns

Bed volume 1 ml or 6 ml

Bed diameter 6.4 mm (1-ml column)
16 mm (6-ml column)

Bed height 30 mm

Max. pressure 1-ml column: 15 bar (218 psi, 1.5 MPa)
6-ml column: 6 bar (87 psi, 0.6 MPa)

Max. flow rate* 1800 cm/h (10 ml/min for 1-ml column)
1800 cm/h (60 ml/min for 6-ml column)

SOURCE 4.6/100 PE columns (Tricorn)^†

Bed volume 1.7 ml

Bed dimensions 4.6 mm × 100 mm

Max. pressure 40 bar (580 psi, 4 MPa)

Max. flow rate* 5 ml/min (1800 cm/h)

* H₂O at 25°C
^†Columns not suitable for use with ÄKTAprime™ plus system.

Four different pH-values were tested for high resolution separation of recombinant protein DnaK.

Click here for more information about Nucleic Acid Purification

http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165403

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稳定性

可以在常温下运输，在25℃，可稳定保存一周。

酶液可反复冻融数次，对酶活无影响。

5.产品信息

产品名称

产品编号

活性

包装

产地

重组肠激酶

EK0801

≥10u/μl

≥50u/μl

100u 1ku 1MU

上海雅心

TECHNICAL SPECIFICATIONS
SOURCE media
Ion exchanger type
SOURCE™ 15Q, 30Q	Quaternary ammonium strong anion
SOURCE™ 15S, 30S	Methyl sulfonate strong cation
Particle size
SOURCE™ 15Q, 15S	15 μm
SOURCE™ 30Q, 30S	30 μm
Matrix	Polystyrene/divinylbenzene
pH stability	2-12 (long term), 1-14 (short term)
Chemical stability	Stable in all common ion exchange buffers
Storage	20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (S)
Storage temperature	4°C to 30°C
RESOURCE™ prepacked columns
Bed volume	1 ml or 6 ml
Bed diameter	6.4 mm (1-ml column) 16 mm (6-ml column)
Bed height	30 mm
Max. pressure	1-ml column: 15 bar (218 psi, 1.5 MPa) 6-ml column: 6 bar (87 psi, 0.6 MPa)
Max. flow rate*	1800 cm/h (10 ml/min for 1-ml column) 1800 cm/h (60 ml/min for 6-ml column)
SOURCE 4.6/100 PE columns (Tricorn)^†
Bed volume	1.7 ml
Bed dimensions	4.6 mm × 100 mm
Max. pressure	40 bar (580 psi, 4 MPa)
Max. flow rate*	5 ml/min (1800 cm/h)
* H₂O at 25°C ^†Columns not suitable for use with ÄKTAprime™ plus system.