PEZZ 18 EXPRESION VECTOR
英文名称： PEZZ 18 EXPRESION VECTOR
型号:null    产品货号： 27-4810-01
价格:请致电：010-57128832,18610462672
品牌: 美国

                
            27-4810-01            PEZZ 18 EXPRESION VECTOR        
        
            28-4013-51            HIS GRAVITRAP KIT        
        
            28-4055-00            GST MULTITRAP 4B        
        
            28-4055-01            GST MULTITRAP FF        
        
            28-9523-59            GST SpinTrap        
        
            28-9523-60            GST GraviTrap        
        
            28-9523-61            GST Buffer Kit        
        
            28-9545-49            PGEX-4T-1        
        
            28-9545-50            PGEX-4T-2        
        
            28-9545-52            PGEX-4T-3        
        
            28-9545-53            PGEX-5X-1        
        
            28-9545-54            PGEX-5X-2        
        
            28-9545-55            PGEX-5X-3        
        
            28-9546-46            PGEX-2TK        
        
            28-9546-48            PGEX-6P-1 VECTOR        
        
            28-9546-50            PGEX-6P-2 VECTOR        
        
            28-9546-51            PGEX-6P-3 VECTOR        
        
            28-9546-53            PGEX-2T 25 UG WI/DI        
        
            28-9546-54            PGEX-3X 25 UG WI/DI        
        
            28-9546-56            PGEX-1LT ECOR I/BAP        
        
            11-0033-99            HIS GRAVITRAP        
        
            11-0034-00            HIS BUFFER KIT        
        
            27-4710-01            ANTI-HIS ANTIBODY        
        
            28-4009-89            HIS MULTITRAP HP        
        
            28-4009-90            HIS MULTITRAP FF        
        
            28-4013-53            HIS SPINTRAP        
        
            28-4039-43            COLLECTION PLATE        
        
            28-9321-71            His SpinTrap Kit        
        
            27-1583-01            LIGHT PRIMER MIX        
    
pEZZ 18 Protein A Gene Fusion Vector
pEZZ 18 Protein A Gene Fusion Vector
        
  • For rapid expression of secreted fusion proteins and their one-step purification using IgG Sepharose 6 Fast Flow
 
The phagemid pEZZ 18 contains the protein A signal sequence and two synthetic “Z” domains based on the “B” IgG binding domain of protein A. Proteins are expressed as fusions with the “ZZ” peptide and secreted into the aqueous culture medium under the direction of the protein A signal sequence. They are easily purified using IgG Sepharose 6 Fast Flow to which the “ZZ” domain binds tightly. Because of its folding properties, the 14 kDa “ZZ” peptide has little effect on folding of the fusion partner into a native conformation.
Expression
Expression is controlled by the lacUV5 and protein A promoters and is not inducible. Elements of the protein A gene provide the ATG and ribosome-binding sites. Stop codons must be provided by the insert
Sequencing
M13 Universal Sequencing Primer can be used for both double-stranded and single-stranded sequencing. A protocol for production of single-stranded DNA is provided with the vector
Cloning
Inserts containing a stop codon will yield white colonies when grown on media containing X-gal
Host(s)
E. coli strains carrying a lac deletion but capable of 
a-complementation of lacZ’.
pEZZ 18 Protein A Gene Fusion Vector — Technical Specifications
            
            Complete Packsize            25 µg        
        
            Order Information            For research use only. Not for diagnostic or therapeutic purposes.        
        
            Selectable Marker [s]            Plasmid confers resistance to 70 μg/ml ampicillin        
        
            
                        
Licensing
            		                        
For licensing information, see the Licensing Statements page in the About Us section