17-6000-86 | IPG-BUFFER, PH 4-7L | 17-6000-87 | IPG-BUFFER, PH 3-10L | 17-6000-88 | IPG-BUFFER, PH 3-10NL | 17-6001-10 | IMMOBILINE DRYSTRIP PH 4-7,7CM | 17-6001-11 | IMMOBILINE DRYSTRIP PH3-10,7CM | 17-6001-12 | IMMOBILINE DRYSTRIP PH 3-10NL, | 17-6001-13 | IMMOBILINE DRYSTRIP PH4-7, 13C | 17-6001-14 | IMMOBILINE DRYSTRIPPH3-10,13CM | 17-6001-15 | IMMOBILINE DRYSTRIP PH3-10NL, | 17-6001-78 | IPG BUFFER, PH 6-11 | 17-6001-88 | IMMOBILINE DRYSTRIP PH 6-9,18C | 17-6001-94 | IMMOBILINE DRYSTRIP PH 6-11,7C | 17-6001-95 | IMMOBILINE DRYSTRIP PH 6-11,11 | 17-6001-96 | IMMOBILINE DRYSTRIP PH 6-11,13 | 17-6001-97 | IMMOBILINE DRYSTRIP PH 6-11,18 | 17-6002-02 | IPG BUFFER, PH 3-5.6 NL | 17-6002-04 | IPG BUFFER, PH 4.5-5.5 | 17-6002-05 | IPG BUFFER, PH 5.0-6.0 | 17-6002-06 | IPG BUFFER, PH 5.3-6.5 | IPG Buffers - For use with Immobiline™ DryStrip gels to improve protein solubility.
- Using Immobiline™ DryStrip gels together with matching IPG Buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.
- Improved IPG Buffer pH 3-10 and pH 3-10 NL offer improved resolution, sensitivity, and increased spot intensity as well as additional protein spots in high molecular weight and basic pH areas.
- Eliminates background staining.
IPG BuffersTechnical Information
IPG Buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. IPG Buffer pH 3-10 and pH 3-10 NL have been improved with a new formula to increase resolution, sensitivity, and spot intensity.
Each IPG Buffer produces a more uniform conductivity along the Immobiline DryStrip during focusing, resulting in wider latitude in run times. IPG Buffers also eliminate high background staining. IPG Buffers are supplied in 1 ml aliquots of water. The buffer is diluted 50- or 200-fold in the rehydration solution, depending on the first-dimension system and pH range of the strip.
TECHNICAL SPECIFICATIONS | Content | Specialized carrier ampholytes in aqueous solution | Volume | 1 ml | Storage | 4°C to 8°C | Shelf life | 36 months | The basic area of 2-D protein spot maps using Immobiline™ DryStrip pH 3-10 NL (18 cm) together with A) improved IPG Buffer pH 3-10 NL or B) the previous recipe for IPG Buffer pH 3-10 NL. The improved IPG Buffer formula results in additional spots, and some of the differences are indicated in the figure.
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165728 |