AlphaScreen?IP3 Assay Supplement, 10,000 assay points
型号:null    产品货号： 6760621M
价格:请致电：010-57128832,18610462672
品牌: perkinelmer

Detection of IP3 with AlphaScreen is based on the competition between IP3 produced by cells and a biotinylated IP3 analog (b-IP3) binding to a GST-tagged IP3 binding protein (GST-IP3 -bp). The b-IP3 and GST-IP3 bp are recognized by the streptavidin-Donor

-580px.jpg" target="_blank" style="color: rgb(0, 64, 159); text-decoration: none;">Migration patterns for PageRuler Prestained Protein Ladder. Migration patterns of PageRuler Plus Prestained Protein Ladder in different electrophoretic conditions. The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.

References:

  1. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680-685.
  2. Burnette, W.N. (1981). Western blotting: electrophoretic transfer of proteins from sodium dodecyl sulfate – polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A, Anal. Biochem., 112 (2), 195-203.
  3. Towbin, H., et al. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc. Natl. Acad. Sci. USA, 76, 4350-4354.

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