17-5166-01
            
MONO Q 5/50 GL
        
        
            
17-5167-01
            
MONO Q 10/100 GL
        
    
MonoBeads™ Columns
Technical Information

MonoBeads™ are prepacked in Tricorn™, HR, and PC column formats. The three Tricorn™ (5/50 GL, 10/100 GL, and 4.6/100 PE) columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads™ columns (see page 511).
 
Tricorn™ columns are ideal for the polishing step when high purity is required. HR 16/10 columns, on the other hand, have higher loading capacity than Tricorn™ columns. PC 1.6/5 columns are ideal for micropurification of subnanogram to microgram amounts of sample on ÄKTA Systems or other high-performance LC systems.
 
Customer feedback on Mono Q HR 5/5.
 
    
        
            
TECHNICAL SPECIFICATIONS
        
        
            
MonoBeads™ 
        
        
            
Ion exchanger type 
            
Mono Q™ 
            
Strong anion 
        
        
            
 
            
Mono S™ 
            
Strong cation 
        
        
            
Ionic capacity 
            
Mono Q™ 
            
0.27-0.37 mmol Cl-/ml 
        
        
            
 
            
Mono S™ 
            
0.12-0.15 mmol H+/ml 
        
        
            
pH stability 
            
 
            
2-12 (working), 1-14 (short term) 
        
        
            
Chemical stability 
            
 
            
Stable in all common buffers except high concentration oxidizing and reactive agents, DMSO, DMF, and formic acid. 
        
        
            
Medium 
            
 
            
Monodisperse polystyrene/divinyl benzene particles 
        
        
            
Particle size 
            
 
            
10 μm 
        
        
            
Storage 
            
 
            
20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (S) 
        
        
            
Storage temperature 
            
4°C to 30°C 
        
        
            
MonoBeads Columns*
        
        
            
Bed volume 
            
PC 1.6/5 
            
0.1 ml 
        
        
            
 
            
5/50 GL 
            
1 ml 
        
        
            
 
            
4.6/100 PE 
            
1.7 ml 
        
        
            
 
            
10/100 GL 
            
8 ml 
        
        
            
 
            
HR 16/10 
            
20 ml 
        
        
            
Max. pressure 
            
PC 1.6/5 
            
50 bar (725 psi, 5 MPa) 
        
        
            
 
            
5/50 GL, 10/100 GL,
             4.6/100 PE 
            
40 bar (580 psi, 4 MPa) 
        
        
            
 
            
HR 16/10 
            
30 bar (435 psi, 3 MPa) 
        
        
            
Recommended flow rate 
            
PC 1.6/5 
            
0.01-0.4 ml/min 
        
        
            
 
            
5/50 GL, 4.6/100 PE 
            
0.5-3.0 ml/min 
        
        
            
 
            
10/100 GL 
            
2.0-6.0 ml/min 
        
        
            
 
            
HR 16/10 
            
< 10 ml/min 
        
        
            
* Columns are not suitable for use with ÄKTAprime™ plus system.
                        
 
            
        
    
 
 
Tricorn high-performance columns are prepacked with MonoBeads ion exchange media for high-resolution purification and high capacity.
 
 
The polishing step on Mono Q 5/50 GL shows different phosphoforms of the purified kinase and very high purity.
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165400