17-0110-01 SEPHAROSE 6B   1 L

17-0120-01 SEPHAROSE 4B   1 L

17-0130-01 SEPHAROSE 2B   1 L

17-0140-01 SEPHAROSE CL-2B   1 L

17-0150-01 SEPHAROSE CL-4B   1 L

17-0160-01 SEPHAROSE CL-6B   1 L

17-0360-01 BLUE DEXTRAN 2000   10 G

17-0489-01 SUPEROSE 6 PREP GRADE 125 ML

17-0536-01 SUPEROSE 12 PREP GRADE 125 ML

17-0584-01 SEPHACRYL S-200 HR 750 ML

17-0584-10 SEPHACRYL S-200 HR, 150 ML

17-0599-01 SEPHACRYL S-300 HR, 750 ML

17-0599-10 SEPHACRYL S-300 HR, 150 ML

17-0609-01 SEPHACRYL S-400 HR, 750ML

17-0609-10 SEPHACRYL S-400 HR, 150 ML

17-0612-01 SEPHACRYL S-100 HR 750ML

17-0612-10 SEPHACRYL S-100 HR, 150 ML

17-0613-01 SEPHACRYL S-500 HR   750ML

17-0613-10 SEPHACRYL S-500 HR, 150 ML

17-0905-01 SUPERDEX 30 PREP GRADE 150 ML

17-1043-01 SUPERDEX 200 PREP GRADE 150 ML

17-1044-01 SUPERDEX 75 PREP GRADE 150 ML

18-1022-18 PM GEL FILTRATION,PRINC.& METH

18-1132-29 CIPP HANDBOOK

18-1165-33 COLUMN PACKING THE MOVIE CD

28-4038-41 GEL FILTRATION CAL KIT LMW

28-4038-42 GEL FILTRATION CAL KIT HMW

Sepharose 4B

Sepharose 4B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

4% Agarose gel filtration media.
Proven base matrix for coupling affinity ligands

Sepharose is a bead-formed agarose-based gel filtration matrix. Sepharose is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose 2B, Sepharose 4B and Sepharose 6B respectively, Both Sepharose and Sepharose CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight

Sepharose CL gels are cross-linked derivatives of Sepharose 2B, Sepharose 4B and Sepharose 6B. The cross-linked form of Sepharose is chemically and physically more resistant than Sepharose itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose gels are resistant to organic solvents and are thus the choice for separations in organic solvents

Sepharose 4B — Technical Specifications

Matrix 4% agarose

Particle Size 45 μm-165 μm

pH stability Working Range 4-9

pH stability Cleaning-in-Place (CIP) 4-9

Pressure/Flow Specification 70-140 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.

Flow Velocity <11.5 cm/h

Fractionation [Mr] Globular Proteins 6 x 10⁴-2 x 10⁷

Exclusion Limit > 2 x 10⁷

Storage Conditions 4 to 30°C, 20% Ethanol