17-0757-01
            
PREPACKED GLUTATHION SEPHAROSE
        
        
            
18-1128-14
            
DF GST 96-WELL DETECTION MODUL
        
        
            
18-1157-58
            
GST SYSTEM HANDBOOK
        
        
            
27-0843-01
            
PRESCISSION PROTEASE
        
        
            
27-0846-01
            
THROMBIN PROTEASE
        
        
            
27-0849-01
            
FACTOR XA
        
        
            
27-1410-01
            
5 PGEX SEQUENCING PRIMER
        
        
            
27-1411-01
            
3 PGEX SEQUENCING PRIMER
        
        
            
27-1524-01
            
M13K07     WI    1 V
        
        
            
27-1542-01
            
E. COLI BL21
        
        
            
27-4570-01
            
GST Bulk Kit
        
        
            
27-4577-01
            
ANTI-GST ANTIBODY
        
        
            
27-4577-50
            
ANTI-GST ANTIBODY, 50MG
        
        
            
27-4583-01
            
PGEX-4T-3
        
        
            
27-4590-01
            
GST DETECTION MODULE
        
        
            
27-4810-01
            
PEZZ 18 EXPRESION VECTOR
        
        
            
28-4013-51
            
HIS GRAVITRAP KIT
        
        
            
28-4055-00
            
GST MULTITRAP 4B
        
        
            
28-4055-01
            
GST MULTITRAP FF
        
        
            
28-9523-59
            
GST SpinTrap
        
        
            
28-9523-60
            
GST GraviTrap
        
        
            
28-9523-61
            
GST Buffer Kit
        
    
Glutathione Sepharose™ 4B Media/GSTrap 4B Columns - Easy Purification
    
Fast and simple purification of GST*-tagged proteins.
    
GSTrap™ 4B 1-ml and 5-ml columns are prepacked with Glutathione Sepharose™ 4B.
    
GSTrap™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTAdesign.
Glutathione Sepharose™ 4B Media/GSTrap 4B Columns - Easy Purification
Technical Information

 
GSTrap 4B 1-ml and 5-ml columns are prepacked with Glutathione Sepharose 4B for fast purification of GST-tagged proteins and scale-up.
 
GSTrap™ 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function.
 
For an introduction to Glutathione S-transferase (GST) Gene Fusion System, see Introduction to Glutathione S-Transferase (GST) Gene Fusion System.
 
 
    
        
            
Columns: 
            
Capture   GSTrap 4B 1 ml
            Polishing   HiLoad 16/60 Superdex 200 pg
        
        
            
Sample:
            
Cell extract from E. coli expressing GST-hippocalcin (Mr 45 000) 
            after lysis with CelLytic Express
        
        
            
AC binding/wash buffer: 
            
10 mM sodium phosphate, 140 mM, pH 7.4
        
        
            
AC elution buffer: 
            
50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0
        
        
            
GF buffer: 
            
50 mM phosphate buffer, 150 mM NaCl, pH 7.2
        
        
            
System:
            
ÄKTAprime plus
        
    
 
 
 
1 Low molecular weight markers
2 Start material (diluted 1:20)
3 Eluted pool GSTrap 4B
4 Eluted pool GSTrap 4B (diluted 1:50)
5 Peak 1, HiLoad 16/60 Superdex 200 pg
6 Peak 2, HiLoad 16/60 Superdex 200 pg
 
    
        
            
TECHNICAL SPECIFICATIONS
        
        
            
Ligand 
            
Glutathione 
        
        
            
Ligand coupling method 
            
Epoxy activation 
        
        
            
Dynamic binding capacity* 
            
> 5 mg horse liver GST/ml medium 
        
        
            
Matrix 
            
Agarose, 4% 
        
        
            
Average particle size 
            
90 μm 
        
        
            
pH stability 
            
4-13 
        
        
            
Storage 
            
20% ethanol 
        
        
            
Storage temperature 
            
4°C to 30°C 
        
        
            
HiTrap prepacked columns
        
        
            
Column volume 
            
1 ml and 5 ml 
        
        
            
Column dimensions 
            
0.7 × 2.5 cm (1 ml); 1.6 × 2.5 cm (5 ml)
             
        
        
            
Recommended flow rate† 
            
0.2-1 ml/min (1 ml); 0.5-5 ml/min (5 ml)
             
        
        
            
Max. pressure† 
            
3 bar (0.3 MPa) 
        
        
            
* Binding of GST to glutathione is flow-rate dependent and lower flow rates often iincrease the binding capacity. This is an important consideration, especially during sample loading and elution.
            † H2O at 25°C.