英文名称： SEPHACRYL S-200 HR
| 17-0010-01 | SEPHADEX G-10 100 G |
| 17-0010-02 | SEPHADEX G-10 500 G |
| 17-0020-01 | SEPHADEX G-15 100 G |
| 17-0020-02 | SEPHADEX G-15 500 G |
| 17-0031-01 | SEPHADEX G-25 SUPERFINE 100 |
| 17-0032-01 | SEPHADEX G-25 FINE 100 G |
| 17-0032-02 | SEPHADEX G-25 FINE 500 G |
| 17-0033-01 | SEPHADEX G-25 MEDIUM 100 G |
| 17-0033-02 | SEPHADEX G-25 MEDIUM 500 G |
| 17-0034-01 | SEPHADEX G-25 COARSE 100 G |
| 17-0034-02 | SEPHADEX G-25 COARSE 500 G |
| 17-0041-01 | SEPHADEX G-50 SUPERFINE 100 |
| 17-0042-01 | SEPHADEX G-50 FINE 100 G |
| 17-0042-02 | SEPHADEX G-50 FINE 500 G |
| 17-0043-01 | SEPHADEX G-50 MEDIUM 100 G |
| 17-0043-02 | SEPHADEX G-50 MEDIUM 500 G |
| 17-0044-01 | SEPHADEX G-50 COARSE 100 G |
| 17-0044-02 | SEPHADEX G-50 COARSE 500 G |
| 17-0050-01 | SEPHADEX G-75 100 G |
| 17-0051-01 | SEPHADEX G-75 SUPERFINE 100 |
| 17-0060-01 | SEPHADEX G-100 100 G |
| 17-0060-02 | SEPHADEX G-100 500 G |
| 17-0061-01 | SEPHADEX G-100 SUPERFINE 100 |
| 17-0090-01 | SEPHADEX LH-20 100 G |
| 17-0090-02 | SEPHADEX LH-20 500 G |
| 17-0090-10 | SEPHADEX LH-20, 25 G |
| 17-0110-01 | SEPHAROSE 6B 1 L |
| 17-0120-01 | SEPHAROSE 4B 1 L |
| 17-0130-01 | SEPHAROSE 2B 1 L |
| 17-0140-01 | SEPHAROSE CL-2B 1 L |
| 17-0150-01 | SEPHAROSE CL-4B 1 L |
| 17-0160-01 | SEPHAROSE CL-6B 1 L |
| 17-0360-01 | BLUE DEXTRAN 2000 10 G |
| 17-0489-01 | SUPEROSE 6 PREP GRADE 125 ML |
| 17-0536-01 | SUPEROSE 12 PREP GRADE 125 ML |
| 17-0584-01 | SEPHACRYL S-200 HR 750 ML |
| 17-0584-10 | SEPHACRYL S-200 HR, 150 ML |
| 17-0599-01 | SEPHACRYL S-300 HR, 750 ML |
| 17-0599-10 | SEPHACRYL S-300 HR, 150 ML |
| 17-0609-01 | SEPHACRYL S-400 HR, 750ML |
| 17-0609-10 | SEPHACRYL S-400 HR, 150 ML |
| 17-0612-01 | SEPHACRYL S-100 HR 750ML |
| 17-0612-10 | SEPHACRYL S-100 HR, 150 ML |
| 17-0613-01 | SEPHACRYL S-500 HR 750ML |
| 17-0613-10 | SEPHACRYL S-500 HR, 150 ML |
| 17-0905-01 | SUPERDEX 30 PREP GRADE 150 ML |
| 17-1043-01 | SUPERDEX 200 PREP GRADE 150 ML |
| 17-1044-01 | SUPERDEX 75 PREP GRADE 150 ML |
HiPrep™ Sephacryl™ HR Columns and Sephacryl™ Lab Packs
- Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl ***/ bisacrylamide matrix.
- Sephacryl™ S-100 HR is designed for separating peptides and small proteins.
- S-200 HR and S-300 HR are for purifying antibodies, serum proteins, and mid-size proteins.
- S-400 HR and S-500 HR are used to separate polysaccharides, macromolecules with extended structures, and even small plasmids.
- S-1000 SF is optimized for the purification of ***s up to 108 molecular weight, spherical particles up to 400 nm, and DNA up to 20 000 base pairs including plasmids, vesicles, and viruses.
- Convenient, prepacked HiPrep™ Sephacryl™ columns speed up protein purification
HiPrep™ Sephacryl™ HR Columns and Sephacryl™ Lab Packs
Technical Information
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HiPrep™ Sephacryl™ HR columns, prepacked with Sephacryl™ High Resolution media, provide reliable gel filtration separations.
HiPrep™ Sephacryl™ HR prepacked gel filtration columns offer excellent separation properties in a convenient, ready-to-use format. By combining the performance of Sephacryl™ High Resolution media with a low cost column construction, they are both reliable and cost effective to use with simple equipment or systems.
Five HiPrep™ Sephacryl™ HR columns are available prepacked with S-100, S-200, S-300, S-400, or S-500 HR media, respectively. Each column is also available in two sizes: 16/60 for sample volumes of up to 5 ml and 26/60 for sample volumes of up to 13 ml. The range of HiPrep™ Sephacryl™ HR prepacked columns allows purification of molecules with molecular weights as small as 1 × 103 to macromolecules as large as 2 × 107.
| TECHNICAL SPECIFICATIONS | |
| Sephacryl™ High Resolution | |
| Fractionation range (Mr) - | |
| - Globular proteins | |
| S-100 HR S-200 HR S-300 HR S-400 HR | 1 × 103 - 1 × 105 5 × 103 - 2.5 × 105 1 × 104 - 1.5 × 106 2 × 104 - 8 × 106 |
| - Dextrans | |
| S-200 HR S-300 HR S-400 HR S-500 HR S-1000 SF | 1 × 103 - 8 × 104 2 × 103 - 4 × 105 1 × 104 - 2 × 106 4 × 104 - 2 × 107 5 × 105 - > 108 |
| Exclusion limit up to 400 nm for spherical particles. | |
| - DNA exclusion limit | |
| S-200 HR S-300 HP S-400 HR S-500 HR S-1000 SF | 30 bp 118 bp 271 bp 1078 bp 20 000 bp |
| Matrix | Spherical allyl *** and N, N’-methylenebisacrylamide |
| Chemical stability | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine-HCl, 1% SDS, 2 M NaCl, 24% ethanol, 30% propanol, 30% acetonitrile, 0.5 M NaOH (for cleaning) |
| pH stability | 3-11 (long term and working) 2-13 (short term) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| Physical stability Autoclavability Antimicrobial agent& | |