英文名称： CM SEPHAROSE FAST FLOW
| 17-0146-01 | SOURCE 15ETH, 50 ML |
| 17-0147-01 | SOURCE 15PHE, 50 ML |
| 17-0148-01 | SOURCE 15ISO, 50 ML |
| 17-0170-01 | DEAE-SEPHADEX A-25 100 G |
| 17-0170-02 | DEAE-SEPHADEX A-25 500 G |
| 17-0180-01 | DEAE-SEPHADEX A-50 100 G |
| 17-0180-02 | DEAE-SEPHADEX A-50 500 G |
| 17-0190-01 | QAE-SEPHADEX A-25 100 G |
| 17-0200-01 | QAE-SEPHADEX A-50 100 G |
| 17-0210-01 | CM-SEPHADEX C-25 100 G |
| 17-0210-02 | CM-SEPHADEX C-25 500 G |
| 17-0220-01 | CM-SEPHADEX C-50 100 G |
| 17-0220-02 | CM-SEPHADEX C-50 500 G |
| 17-0230-01 | SP-SEPHADEX C-25 100 G |
| 17-0240-01 | SP-SEPHADEX C-50 100 G |
| 17-0500-01 | DEAE-SEPHACEL 500 ML |
| 17-0510-01 | Q SEPHAROSE FAST FLOW 300 ML |
| 17-0510-10 | Q SEPHAROSE FAST FLOW, 25 ML |
| 17-0709-01 | DEAE SEPHAROSE FAST FLOW 500ML |
| 17-0709-10 | DEAE SEPHAROSE FAST FLOW, 25ML |
| 17-0710-01 | DEAE-SEPHAROSE CL-6B 500 ML |
| 17-0712-01 | PBE 94, 200ML |
| 17-0713-01 | POLYBUFFER 74 250ML |
| 17-0714-01 | POLYBUFFER 96 250ML |
| 17-0719-01 | CM SEPHAROSE FAST FLOW 500ML |
| 17-0719-10 | CM SEPHAROSE FAST FLOW, 25 ML |
| 17-0729-01 | SP SEPHAROSE FAST FLOW, 300 ML |
| 17-0729-10 | SP SEPHAROSE FAST FLOW, 25 ML |
| 17-0790-01 | OCTYL-SEPHAROSE CL-4B 200 ML |
| 17-0810-01 | PHENYL-SEPHAROSE CL-4B 200 ML |
| 17-0944-01 | SOURCE 15S, 50 ML |
| 17-0944-10 | SOURCE 15S, 10 ML |
| 17-0946-02 | OCTYL SEPHAROSE 4 FAST FLOW 200ML |
| 17-0946-10 | OCTYL SEPHAROSE 4FAST FLOW, 25ML |
| 17-0947-01 | SOURCE 15Q, 50 ML |
| 17-0947-20 | SOURCE 15Q, 10 ML |
| 17-0965-05 | PHENYL SEPHAROSE 6 FF LS 200 ML |
| 17-0965-10 | PHENYL SEPHAROSE 6 FF / LS, 25ML |
| 17-0973-05 | PHENYL SEPHAROSE 6FF (HS) 200ML |
| 17-0973-10 | PHENYL SEPHAROSE 6 FF /HS, 25ML |
IEX Sepharose™ Fast Flow
- For preparative purifications, even when purifying crude samples.
- Available with strong (Q and SP) and weak (DEAE, CM, and ANX) anion and cation exchangers, in bulk quantities and in prepacked HiTrap™ and HiPrep™ columns, as well as HiTrap™ IEX Selection Kit.
- For separation of proteins of all pI values with Q and SP having a high dynamic capacity for proteins over the entire pH range.
- ANX has a different selectivity compared to DEAE, and is applicable for separation of high molecular weight proteins.
- Easy scale-up from HiTrap™ scale to laboratory scale to process scale.
IEX Sepharose™ Fast Flow
Technical Information
| TECHNICAL SPECIFICATIONS | |
| Ion exchanger type | |
| Q Sepharose™ FF | Quaternary ammonium strong anion |
| DEAE Sepharose™ FF | Diethylaminoethyl weak anion |
| ANX Sepharose™ 4 FF | Diethylaminopropyl weak anion |
| SP Sepharose™ FF | Sulfopropyl strong cation |
| CM Sepharose™ FF | Carboxymethyl weak cation |
| Ionic capacity | |
| Q Sepharose™ FF | 0.18-0.25 mmol (Cl-)/ml |
| DEAE Sepharose™ FF | 0.11-0.16 mmol (Cl-)/ml |
| ANX Sepharose™ 4 FF | 0.13-0.18 mmol (Cl-)/ml |
| SP Sepharose™ FF | 0.18-0.25 mmol (H+)/ml |
| CM Sepharose™ FF | 0.09-0.13 mmol (H+)/ml |
| Dynamic capacity | |
| Q Sepharose™ FF | 120 mg HSA/ml medium |
| DEAE Sepharose™ FF | 110 mg HSA/ml medium |
| ANX Sepharose™ 4 FF | 5 mg thyroglobulin/ml medium |
| SP Sepharose™ FF | 70 mg RNase/ml medium |
| CM Sepharose™ FF | 50 mg RNase/ml medium |
| Pressure/flow spec. | |
| Q Sepharose™ FF | 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm |
| DEAE Sepharose™ FF | 300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm |
| ANX Sepharose™ 4 FF | min 200 cm/h, 100 kPa, XK50/60 column, bed height 25 cm |
| SP Sepharose™ FF | 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm |
| CM Sepharose™ FF | 300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm |
| Average particle size | 90 μm (45-165 μm) |
| Matrix | |
| Sepharose™ FF | Highly cross-linked agarose, 6% |
| Sepharose™ 4 FF | Highly cross-linked agarose, 4% |
| pH stability | |
| Q Sepharose™ FF | 1-14 (short term), 2-12 (long term) |
| DEAE Sepharose™ FF | 1-14 (short term), 2-13 (long term) |
| ANX Sepharose™ 4 FF | 2-14 (short term), 3-13 (long term) |
| SP Sepharose™ FF | 3-14 (short term), 4-13 (long term) |
| CM Sepharose™ FF | 2-14 (short term), 4-13 (long term) |
| Chemical stability | Stable in all common aqueous buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH*, and 1 M acetic acid*. |
| Storage | 20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP) |
| Storage temperature | 4°C to 30°C |
| *1 M NaOH and acetic acid should be used only for cleaning purposes.
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Recombinant human superoxide dismutase produced by Saccharomyces cerevisiae is purified directly from clarified cell homogenate on Q Sepharose™ Fast Flow after desalting and buffer exchange on Sephadex™ G-25. A 2.6 × 5 ml ion exchange column handles 390 ml of sample in 45 min.
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