HIGHLIGHTS • Simple, rapid recovery of ultra-pure DNA from PCR, endonuclease digestions, “crude” DNA preps., etc. • Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer. Unique column construction allows sample washing to be performed using a centrifuge, vacuum source, or syringe. • Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer using a microcentrifuge. • Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc.

SPECIFICATIONS  • Format: Spin Column. • DNA Purity: Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. • DNA Size Limits: From 50 bp to 23 kb. • DNA Recovery: Typically, ≤ 100 µg total DNA can be eluted with ≥ 100 µl water or low salt elution buffer. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%. • Sample Sources: DNA from PCR, restriction endonuclease digestions, “crude” DNA preparations, etc. • Product Detergent Tolerance: ≤ 5% Triton X-100, ≤ 5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS. • Equipment: Microcentrifuge and centrifuge, vacuum source, or syringe.

DESCRIPTION The DNA Clean & Concentrator™-100 was designed for the rapid purification and concentration of up to 100 µg of high quality DNA from PCR, large format restriction endonuclease digestions, or "crude" DNA preparations. The DCC™-100 features a single-buffer system that allows for efficient DNA adsorption onto the matrix of the supplied Zymo-Spin™ column. The DNA is washed twice using either a centrifuge, vacuum source, or syringe then eluted with a small volume (≥ 100 µl) of water using a microcentrifuge. The procedure is easy: simply add the specially formulated DNA Binding Buffer to your sample then transfer the mixture to the supplied to your sample then transfer the mixture to the supplied Zymo-Spin™ column. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin column technology to yield high quality, purified DNA in just minutes. DNA purified using the DCC™-100 is suitable for DNA sequencing, DNA transfection, RNA transcription, microarray analysis, PCR, nucleotide blotting, restriction endonuclease digestion, etc.   DNA sequencing chromatogram of DNA isolated using the DNA Clean & Concentrator™.

ORDERING INFORMATION: Products Description Protocol Cat No. Size    Price   (RMB)   DNA Clean & Concentrator™-100 For 25 DNA preps. PDF D4029 1 kit     For 50 DNA preps. PDF D4030 1 kit