EASYspin Plus 多糖多酚/复杂植物RNA快速提取试剂盒 英文名称: EASYspin Plus 多糖多酚/复杂植物RNA快速提取试剂盒 |
型号:null 产品货号: RN5301 |
价格:请致电:010-57128832,18610462672 |
品牌: 国产 |
本公司独家推出EASYspin无***酚、氯仿RNA快速提取技术基础上,又独家研发成功基因组DNA清除柱技术可以有效清除gDNA残留,得到的RNA一般不需要DNase消化,可用于反转录PCR、荧光定量PCR等实验。独特的裂解液迅速裂解细胞和灭活细胞RNA酶, 离心沉淀去除多糖多酚和次级代谢产物,然后裂解混合物用乙醇调节RNA结合吸附到基因组DNA清除柱,然后RNA被选择性洗脱滤过, 吸附在基因组DNA清除柱上的残留DNA无法洗脱连同柱子一起丢弃从而清除掉DNA。滤过的RNA用乙醇调节结合条件后,RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜,再通过一系列快速的漂洗-离心的步骤,去蛋白液和漂洗液将细胞代谢物,蛋白等杂质去除, 最后低盐的RNase free H20将纯净RNA从硅基质膜上洗脱。 产品特点: 1.完全不使用***酚,氯仿等试剂,也不需要乙醇沉淀等步骤。 1. 桃果实、花、根、叶:Isolation, characterisation and phylogenetic analysis of resistance gene analogues in a wild species of peach (Prunus kansuensis).Canadian Journal of Plant Science, 2011, 91(6): 961-970 2. 樱桃花、叶、颚等各部位:Over-expression of the PaAP1 gene from sweet cherry (Prunus avium L.) causes early floweri.Journal of Plant Physiology, 2012,Available online 1 December 2012 3. 洋葱根、茎、蕾、叶、雌雄蕊等各部位:Cloning and Expression Analysis of A Putative B Class MADS-box Gene of AcPI in Onion. 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