DIG Oligonucleotide Tailing Kit,寡核苷酸加尾地高辛标记试剂盒
英文名称： DIG Oligonucleotide Tailing Kit
型号:null    产品货号： 3353583910
价格:请致电：010-57128832,18610462672
品牌: roche

DIG Oligonucleotide Tailing Kit,寡核苷酸加尾地高辛标记试剂盒，通过末端转移酶以DIG-11-ddUTP在寡核苷酸探针3’端加一平均长度50bp(10-100bp)的寡核苷酸, 用于DNA/RNA膜杂交和原位杂交。每个反应可标记100pmol的寡聚核苷酸。

Application

Oligonucleotides, tailed with DIG-dUTP, are used for DNA or RNA hybridization in membrane blots or in situ hybridization. 

Benefits

  • Very sensitive hybridization probes, due to the incorporation of several DIG-nucleotides
  • Fast hybridization kinetics, due to the small size of oligonucleotides
  • Single-stranded probes, no renaturation during hybridization
  • Sequence can be designed according to the experiment
  • Specially suited for in situ hybridization; due to their small size, oligonucleotides readily diffuse into fixed tissues and cells 

Product Description

Tailing of oligonucleotides at the 3-end with DIG-11-dUTP and recombinant Terminal Transferase. Oligonucleotides are tailed with DIG-dUTP and dATP at an average tail length of 50 nucleotides (tail length range: 10 – 100). 

Contents

  1. Reaction Buffer, 5x, 100 µl
  2. CoCl2 Solution, 100 µl, 25 mM
  3. DIG-dUTP Solution, 25 µl, 1 mM
  4. dATP Solution, 25 µl, 10 mM
  5. Recombinant Terminal Transferase, 25 µl, [400 U/µl]
  6. Control Oligonucleotide, unlabeled, 25 µl, [20 pmol/µl]
  7. Oligonucleotide, DIG-dUTP/dATP tailed, 20 µl, [2.5 pmol/µl]
  8. Control DNA, 20 µl, [0.25 mg/ml]
  9. Glycogen Solution, 50 µl, [20 mg/ml]
  10. DNA Dilution Buffer, 3 x 1 ml, [50 µg/ml] fish sperm DNA
  11. poly(A) Solution, 1 ml, [10 mg/ml] 

Principle

DIG-dUTP and dATP are combined at a concentration that gives the highest DIG incorporation into the tail, and optimal spacing of DIG and dATP, to achieve the highest sensitivity in hybridization experiments. DIG-dUTP and dATP are provided as separate solutions to allow greater flexibility in terms of tail length, hapten spacing, and the use of unlabeled nucleotide(s).

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