pCAMBIA1390-GFP
英文名称: pCAMBIA1390-GFP
型号:null    产品货号: BiovectorPCM1390G
价格:请致电:010-57128832,18610462672
品牌: biovector ntcc inc. usa
试剂级别: 分子生物学级

 

Product Data Sheet

Order ID

Name

Description

BiovectorPCM1390G

pCAMBIA1390-GFP

pCAMBIA1390-GFP, 15uL DNA. KanR.Storage:-20

transformation2_3.pngStandard heat-shock transformation of chemically competent bacteria

  1. Take competent cells out of -80°C and thaw on ice.
  2. Take agar plates (containing the appropriate antibiotic) out of 4°C to warm up to room temperature or place in 37°C incubator.
  3. Mix 5μl of DNA into 100μL competent cells in a microcentrifuge or falcon tube. GENTLY mix by flicking the bottom of the tube with your finger a few times.
  4. Place the competent cell/DNA mixture on ice for 20-30min.
  5. Heat shock each transformation tube by placing the bottom 2/3 of the tube into a 42°C water bath for 42 seconds. Put the tubes back on ice for 2 min.
  6. Add 250-500μl SOC media (without antibiotic) and grow in 37°C shaking incubator for 45min.
  7. Plate all of the transformation onto a 10cm LB agar plate containing the appropriate antibiotic.
  8. Incubate plates at 37°C overnight.

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