Vigene miRNA 克隆产品优势

　　▲pMIR 载体是专门为腺病毒载体骨干同源重组和腺病毒生产而特别设计的
　　▲miRNA质粒克隆据有完全的功能且插入的miRNA可以转移到其他载体
　　▲我们拥有全世界最大的人源miRNA 克隆产品系列
　　▲我们拥有miRBase v.18数据库中1272种即可使用的miRNA 克隆
　　▲除特殊情况,如mir302s，每份克隆只包含一种

Safety Considerations（安全注意事项）

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
• Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
• No eating, drinking or smoking while handling the TLR2 stable line.
• Wash hands after handling the TLR2 stable line and before leaving the lab.
• Decontaminate work surface with disinfectant or 70% ethanol before and after working with cells.
• All waste should be considered hazardous.
• Dispose of all liquid waste after each experiment and treat with bleach.

Figure. 1. Western blot analysis of TLR2 expression in the TLR2 stable cell line using an HA antibody (20 ug total protein/lane). Legend. Vect: Vector/HEK 293 (IMGENEX, IML-200); TLR2: TLR2 stable cell line (IML-202).

Figure 2. Surface expression of TLR2 in the TLR2 stable cell line was analyzed by flow cytometry using a PE-conjugated TLR2 antibody (IMG-416D) and compared with the Vector/HEK 293 control (IML-200). IMGENEX’s Surface TLR Staining Flow Kit (10099K) was used for this test.

Reference（参考文献）
1. Mohsin Md. Syed, Nirmal K. Phulwani, Tammy Kielian. Tumor necrosis factor-alpha (TNF-α) regulates Toll-like receptor 2 (TLR2) expression in microglia. J Neurochem. 2007 November; 103(4): 1461–1471.
2. Allison L. van Lint, Matthew R. Murawski, Rory E. Goodbody, Martina Severa, Katherine A. Fitzgerald, Robert W. Finberg, David M. Knipe, Evelyn A. Kurt-Jones. Herpes Simplex Virus Immediate-Early ICP0 Protein Inhibits Toll-Like Receptor 2-Dependent Inflammatory Responses and NF-κB Signaling. J Virol. 2010 October; 84(20): 10802–10811.
3. Louise Hjerrild Zeuthen, Lisbeth Nielsen Fink, Hanne Frøkiær. Toll-like receptor 2 and nucleotide-binding oligomerization domain-2 play divergent roles in the recognition of gut-derived lactobacilli and bifidobacteria in dendritic cells. Immunology. 2008 August; 124(4): 489–502.
4. Jinkeng Asong, Margreet A. Wolfert, Kaustabh K. Maiti, Douglas Miller, Geert-Jan Boons. Binding and Cellular Activation Studies Reveal That Toll-like Receptor 2 Can Differentially Recognize Peptidoglycan from Gram-positive and Gram-negative Bacteria. J Biol Chem. 2009 March 27; 284(13): 8643–8653.
5. Jian-Wen Dong, Jesus G. Vallejo, Huei-Ping Tzeng, James A Thomas, Douglas L. Mann. Innate immunity mediates myocardial preconditioning through Toll-like receptor 2 and TIRAP-dependent signaling pathways. Am J Physiol Heart Circ Physiol. 2010 March; 298(3): H1079–H1087.
6. Alexandre Jacques, Christian Bleau, Claire Turbide, Nicole Beauchemin, Lucie Lamontagne. Macrophage interleukin-6 and tumour necrosis factor-α are induced by coronavirus fixation to Toll-like receptor 2/heparan sulphate receptors but not carcinoembryonic cell adhesion antigen 1a. Immunology. 2009 September; 128(1pt2): e181–e192.

订购信息：

货号	名称	产地	规格	报价/元	货期
IML-202	TLR2 Stable Cell Line	imgenex	1Vial	13328	2-3周