英文名称： ElectroMAX™ DH10B™ T1 Phage-Resistant Competent Cells
描述
ElectroMAX DH10B T1 Phage-Resistant electrocompetent E. coli cells offer
transformation efficiencies that are among the highest achieved by any
product offered by Life Technologies, and can exceed 1 x 1010 cfu/μg
plasmid DNA in efficiency. They are ideal for applications requiring high
efficiency transformation, including cDNA and gDNA library construction.
Maximizes yield of transformants from limited cloning products
Permits efficient cloning of methylated DNA
Resistant to T1 and T5 bacteriophages
Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
Delivers high yield plasmid preparations for downstream applications
Phage-Resistant Representative Libraries from a Single Transformation
The tonA mutation in DH10B confers resistance to bacteriophages T1 and T5 (1), safeguarding
valuable libraries against contamination. Additionally, mutations in the methylation-dependent
restriction system (mcr
详细说明
常用规格
| Format: | Tube(s)
|
| Species: | E. coli
|
| Plasmid Type: | May be used for plasmids > 20 kb
|
| Product Size: | 0.5 ml
|
| Competent Cell Type: | Electrocompetent
|
| Blue-White Screening: | Yes
|
| Cloning Unstable DNA: | Not Suitable for cloning unstable DNA
|
| Reduces Recombination: | Yes
|
| Cloning Methylated DNA: | Yes |
|
Improves Plasmid Quality:
| Yes |
| Propagating ccdB Vectors:
| Not for ccdB vector propagation
|
| Bacterial or Yeast Strain:
| DH10B
|
| Transformation Efficiency: |
> 1x10^10
|
| F Episome (to make ssDNA):
| Lacks F Episome
|
| Preparing Unmethylated DNA: | Not Suitable for preparing unmethylated DNA |
| T1 Phage - Resistant (tonA):
| Yes
|
| High Throughput Compatibility:
| Not High Throughput-Compatible
|
| Shipping Condition:
| Dry Ice
|
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
内容和储存
Store in ultra-cold freezer (-68 to -85°C).