| 出品公司: | -- |
|---|---|
| 载体名称: | pSH65 |
| 质粒类型: | 酵母基因敲除载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8138 bp |
| 5 测序引物及序列: | -- |
| 3 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 非病毒 |
英文名称： pSH65 酵母基因敲除载体质粒图谱序列抗性
试剂级别： 生化级
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
Plasmid is part of the " DEL-MARKER-SET ". For an individual plasmid order use accession number P30122. Transformed E.coli cells are resistant to ampicillin. The plasmid contains the cre recombinase gene under control of a GAL promoter. Transformed Saccharomyces cerevisiae are resistent to phleomycin. The pSH65 plasmid is usefull to express the cre recombinase under Galactose control. loxP flanked deletion markers will be removed from yeast deletions.
载体序列
LOCUS pSH65 8138 bp DNA circular SYN 15-AUG-2011DEFINITION pSH65 CircularizedACCESSION pSH65KEYWORDS .SOURCE Unknown. ORGANISM Unknown Unclassified.REFERENCE 1 (bases 1 to 8138) AUTHORS Self JOURNAL Unpublished.COMMENT This file is created by Vector NTI http://www.biofeng.com/COMMENT ORIGDB|GenBankCOMMENT VNTAUTHORNAME|biofeng.com|FEATURES Location/Qualifiersmisc_feature 479..1286 /product="ura3 region"CDS 690..1286 /gene="delta-ura3" /product="inactivated ura3 gene"misc_feature complement (2071..2274) /gene="CYC1t" /product="CYC1 terminator of S. cerevisiae"CDS complement (2375..2755) /gene="bleR" /product=“ble gene from Tn5, conferring resistance to bleomycin and phleomycin”;misc_feature complement (2774..3187) /gene="ScTEF1p" /product="TEF1 promoter of S. cerevisiae"misc_feature 3231..3484 /gene="CYC1t" /product="CYC1 terminator of S. cerevisiae"CDS complement (3871..4832) /gene="Cre" /product="encoding of Cre recombinase"CDS complement (4941..5408) /gene="GAL1p" /product="inducible GAL1 promoter, derived from the gene encoding galactokinase"misc_feature 5851..5923 /gene="ori" /product="ColE1 origin of replication"CDS complement (6626..7486) /gene="ampR" /product="bla, encoding of beta-lactamase"misc_feature 7627..1 /gene="CEN6/ARSH4" /product="centromere sequence of chromosome VI (CEN6) plus the autonomously replicating sequence associated with histone 4 (ARSH4)"BASE COUNT 2282 a 1805 c 1925 g 2126 tORIGIN 1 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 61 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt121 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg181 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt241 gcaccatacc acagcttttc aattcaattc atcatttttt ttttattctt ttttttgatt301 tcggtttctt tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa361 ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg421 cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat481 gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct541 atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac601 caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca661 tgtggatatc ttgactgatt tttccatgca tggagggcac agttaagccg ctaaaggcat721 tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac attggtaata781 cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca gacattacga841 atgcacacgg tgtggtgggc ccaggtattg ttagcggttt gaagcaggcg gcagaagaag901 taacaaagga acctagaggc cttttgatgt tagcagaatt gtcatgcaag ggctccctat961 ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa gattttgtta1021 tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat tggttgatta1081 tgacacccgg tgtgggttta gatgacaagg gagacgcatt gggtcaacag tatagaaccg1141 tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga ctatttgcaa1201 agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg gaagcatatt1261 tgagaagatg cggccagcaa aactaaaaaa ctgtattata agtaaatgca tgtatactaa1321 actcacaaat tagagcttca atttaattat atcagttatt accctatgcg gtgtgaaata1381 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt1441 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg1501 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt1561 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct1621 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt1681 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa1741 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc1801 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc1861 tacagggcgc gtcgcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg1921 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa1981 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg2041 cgtaatacga ctcactatag ggcgaattgg gcgtacacgc gtctgtacag aaaaaaaaga2101 aaaatttgaa atataaataa cgttcttaat actaacataa ctataaaaaa ataaataggg2161 acctagactt caggttgtct aactccttcc ttttcggtta gagcggatgt ggggggaggg2221 cgtgaatgta agcgtgacat aactaattac atgatatcga caaaggaaaa gggggacgga2281 tccgtcgagt gggtggtgag cagctcgccg tcgcgcagca ggcgccagcg ctccatccac2341 gcagtgacct caggcggaaa acgggaagac acactcatga gatgcctgca agcaattcgt2401 tctgtatcag gcgcaggagc gtcccgtccg ggtcgaccaa agcggccatc gtgcctcccc2461 actcctgcag ttcgggggca tggatgcgcg gatagccgct gctggtttcc tggatgccga2521 cggatttgca ctgccggtag aactccgcga ggtcgtccag cctcaggcag cagctgaacc2581 aactcgcgag gggatcgagc ccggggtggg cgaagaactc cagcatgaga tccccgcgct2641 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg2701 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga2761 attcgagctc gcccttagat tagattgcta tgctttcttt ctaatgagca agaagtaaaa2821 aaagttgtaa tagaacaaga aaaatgaaac tgaaacttga gaaattgaag accgtttatt2881 aacttaaata tcaatgggag gtcatcgaaa gagaaaaaaa tcaaaaaaaa aaattttcaa2941 gaaaaagaaa cgtgataaaa atttttattg cctttttcga cgaagaaaaa gaaacgaggc3001 ggtctctttt ttcttttcca aacctttagt acgggtaatt aacgacaccc tagaggaaga3061 aagaggggaa atttagtatg ctgtgcttgg gtgttttgaa gtggtacggc gatgcgcgga3121 gtccgagaaa atctggaaga gtaaaaaagg agtagaaaca ttttgaagct atggtgtgtg3181 ggggatcccc gggtaccgag ctcgaattca ctggccgtcg ttttacaacg cggccgcaaa3241 ttaaagcctt cgagcgtccc aaaaccttct caagcaaggt tttcagtata atgttacatg3301 cgtacacgcg tctgtacaga aaaaaaagaa aaatttgaaa tataaataac gttcttaata3361 ctaacataac tataaaaaaa taaataggga cctagacttc aggttgtcta actccttcct3421 tttcggttag agcggatgtg gggggagggc gtgaatgtaa gcgtgacata actaattaca3481 tgactcgagg tcgacaattc caaccttacc caagagttcg ccaaactcag acatcacttt3541 agcaaaaccg cgccgtgctt cttcctcggt ggcattcatc acgaaatgtt cagcactacg3601 catacttttg acaggaaacg caacggatat tgagtcaata tcaggcattc tatcgctcag3661 ctttacagtg acaatgacgg ctggcgactg aatattagtg cttacagaca gcactacata3721 ttttccgtcg atgttgaaat cctttctcat atgtcaccat aaatatcaaa taattatagc3781 aatcatttac gcgttaatgg ctaatcgcca tcttccagca ggcgcaccat tgcccctgtt3841 tcactatcca gggtacggat atagttcatg acaatattta cattggtcca gccaccagct3901 tgcatgatct ccggtattga aactccagcg cgggccatat ctcgcgcggc tccgacacgg3961 gcactgtgtc cagaccaggc caggtatctc tgaccagagt catccttagc gccgtaaatc4021 aatcgatgag ttgcttcaaa aatcccttcc agggcgcgag ttgatagctg gctggtggca4081 gatggcgcgg caacaccatt ttttctgacc cggcaaaaca ggtagttatt cggatcatca4141 gctacaccag agacggaaat ccatcgctcg accagtttag ttacccccag gctaagtgcc4201 ttctctacac ctgcggtgct aaccagcgtt ttcgttctgc caatatggat taacattctc4261 ccaccgtcag tacgtgagat atctttaacc ctgatcctgg caatttcggc tatacgtaac4321 agggtgttat aagcaatccc cagaaatgcc agattacgta tatcctggca gcgatcgcta4381 ttttccatga gtgaacgaac ctggtcgaaa tcagtgcgtt cgaacgctag agcctgtttt4441 gcacgttcac cggcatcaac gttttctttt cggatccgcc gcataaccag tgaaacagca4501 ttgctgtcac ttggtcgtgg cagcccggac cgacgatgaa gcatgtttag ctggcccaaa4561 tgttgctgga tagtttttac tgccagaccg cgcgcctgaa gatatagaag ataatcgcga4621 acatcttcag gttctgcggg aaaccatttc cggttattca acttgcacca tgccgcccac4681 gaccggcaaa cggacagaag cattttccag gtatgctcag aaaacgcctg gcgatccctg4741 aacatgtcca tcaggttctt gcgaacctca tcactcgttg catcgaccgg taatgcaggc4801 aaattttggt gtacggtcag taaattggac atttaacact cagataatgg ttttaagtaa4861 agtgtacagg atcggctctg cccctcgacg gtatcgataa gcttgatatc gaattcctgc4921 agcccggggg atccactagt tctagaatcc ggggtttttt ctccttgacg ttaaagtata4981 gaggtatatt aacaattttt tgttgatact tttattacat ttgaataaga agtaatacaa5041 accgaaaatg ttgaaagtat tagttaaagt ggttatgcag tttttgcatt tatatatctg5101 ttaatagatc aaaaatcatc gcttcgctga ttaattaccc cagaaataag gctaaaaaac5161 taatcgcatt atcatcctat ggttgttaat ttgattcgtt catttgaagg tttgtggggc5221 caggttactg ccaatttttc ctcttcataa ccataaaagc tagtattgta gaatctttat5281 tgttcggagc agtgcggcgc gaggcacatc tgcgtttcag gaacgcgacc ggtgaagacg5341 aggacgcacg gaggagagtc ttccttcgga gggctgtcac ccgctcggcg gcttctaatc5401 cgtactagag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat5461 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatag5521 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa5581 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat5641 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc5701 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg5761 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag5821 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc5881 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag5941 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga6001 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc6061 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg6121 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt6181 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca6241 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca6301 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag6361 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca6421 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg6481 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa6541 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta6601 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag6661 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga6721 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac6781 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc6841 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta6901 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac6961 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat7021 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa7081 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg7141 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag7201 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc7261 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct7321 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat7381 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg7441 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc7501 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta7561 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt7621 ccttttcatc acgtgctata aaaataatta taatttaaat tttttaatat aaatatataa7681 attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt ttccgaagat7741 gtaaaagact ctagggggat cgccaacaaa tactaccttt tatcttgctc ttcctgctct7801 caggtattaa tgccgaattg tttcatcttg tctgtgtaga agaccacaca cgaaaatcct7861 gtgattttac attttactta tcgttaatcg aatgtatatc tatttaatct gcttttcttg7921 tctaataaat atatatgtaa agtacgcttt ttgttgaaat tttttaaacc tttgtttatt7981 tttttttctt cattccgtaa ctcttctacc ttctttattt actttctaaa atccaaatac8041 aaaacataaa aataaataaa cacagagtaa attcccaaat tattccatca ttaaaagata8101 cgaggcgcgt gtaagttaca ggcaagcgat ccgtccta//载体质粒图谱pdf版和相关资料下载
载体应用举例
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