（探针法一步qRT-PCR，提供最高的灵敏度）HiScript II One Step qRT-PCR Probe Kit
英文名称： HiScript II One Step qRT-PCR Probe Kit
型号:null    产品货号： Q222-01
价格:请致电：010-57128832,18610462672
品牌: 江苏南京

 用于探针法qRT-PCR，提供最高的灵敏度

HiScript® II One Step qRT-PCR Probe Kit

 

• 可检测到个位数拷贝的模板或0.1 pg总RNA

• 支持多重探针检测

 

图1. 评价HiScript® II One Step qRT-PCR Probe Kit (Q222)的线性范围及检测灵敏度。

A. 以1 ng-0.1 pg Hela细胞总RNA为模板，扩增RFLPO基因，线性范围达5个数量级，灵敏度可达0.1 pg。

B. 以107-10拷贝体外转录的RNA为模板，扩增HCV基因，线性范围达7个数量级，灵敏度可达10拷贝。

C. 以10-1-10-7稀释度的鸡胚尿囊液RNA为模板，扩增新城疫病毒特异基因，可稳定检测到10-7稀释度。

 

      低拷贝数模板或弱阳性样本的检出率仍然是临床诊断的挑战之一。HiScriptE.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 39.6kDa
Concentration n/a
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues Ser456~Phe765 with two N-terminal Tags, His-tag and T7-tag
 
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
尼克酰胺腺嘌呤二核苷酸***酸氧化酶5(NOX5)重组蛋白The sequence of the target protein is listed below.
SKVTH LLIKRPPFFH YRPGDYLYLN IPTIARYEWH PFTISSAPEQ KDTIWLHIRS QGQWTNRLYE SFKASDPLGR GSKRLSRSVT MRKSQRSSKG SEILLEKHKF CNIKCYIDGP YGTPTRRIFA SEHAVLIGAG IGITPFASIL QSIMYRHQKR KHTCPSCQHS WIEGVQDNMK LHKVDFIWIN RDQRSFEWFV SLLTKLEMDQ AEEAQYGRFL ELHMYMTSAL GKNDMKAIGL QMALDLLANK EKKDSITGLQ TRTQPGRPDW SKVFQKVAAE KKGKVQVFFC GSPALAKVLK GHCEKFGFRF FQENF
USAGE
尼克酰胺腺嘌呤二核苷酸***酸氧化酶5(NOX5)重组蛋白Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)

 

尼克酰胺腺嘌呤二核苷酸***酸氧化酶5(NOX5)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.

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