ZE: 16pt">ApplicationAntigens (preferably in saline) are typically mixed with an equal volume of the adjuvant to form an emulsion. See Harlow, E. and Lane, D., Antibodies a Laboratory Manual, (Cold Spring Harbor Laboratory, 1988) for protocols and information on dosage.Biochem/physiol ActionsThe mycobacteria in Complete Freund′s adjuvant attracts macrophages and other cells to the injection site which enhances the immune response. For this reason, Complete Freund′s Adjuvant is used for initial injections and Incomplete Freund′s Adjuvant for subsequent boosts.