乳果糖检测试剂盒 英文名称: Lactulose Assay Kit |
型号:null 产品货号: K-LACTUL |
价格:请致电:010-57128832,18610462672 |
品牌: 爱尔兰 |
品牌: Megazyme 产品货号:K-LACTUL 品牌:Megazyme 产品品名:乳果糖检测试剂盒 英文品名:Lactulose Assay Kit 规格型号:50 assays For the specific, rapid and sensitive measurement of lactulose in milkand milk-based samples. Reagents included in this kit may also beprepared for use in the procedure described by ISO Method 11285:2004. INTRODUCTION: Thermal treatment of milk is an essential process in the dairy industry. Various heating regimens can be employed, and thus in some cases it becomes necessary to determine thermal history accurately. This can be achieved by quantitative measurement of lactulose, a disaccharide comprising D-galactose and D-fructose that is only produced from the common milk sugar lactose at elevated temperatures. In raw milk the level of lactulose is very low. However, as heat treatment increases this sugar can eventually reach ~ 2 g/L (in the case of UHT milk for example). This kit (K-LACTUL) is suitable for the measurement of lactulose in milk-based products such as fresh milk, UHT milk, evaporated milk and powdered milk. However, this kit is unsuitable for the measurement of lactulose in samples that have been artificially sweetened, such as condensed milk, where the “free fructose” to lactulose ratio is extremely high. Reagents included in this kit can be prepared for use in the measurement of lactulose using the procedure as described by ISO Method 11285:2004. However, the slightly modified advanced format K-LACTUL (Rapid Method) developed by Megazyme, that is both more sensitive and rapid, features an additional enzyme, 6-phosphogluconate dehydrogenase, which results in the sensitivity of the method being improved 2-fold. Additionally, optimised selection and use of highly purified enzymes results in a method that can easily be performed within one working day. KITS: Kits suitable for performing 50 assays are available from Megazyme. The kits contain the full assay method plus: Bottle 1: β-Galactosidase suspension (3 mL). Stable for > 2 years at 4°C. Bottle 2: Glucose oxidase and catalase, lyophilised powder. Stable for > 2 years at -20°C. Bottle 3: Buffer (6 mL, pH 7.6) plus sodium azide (0.02% w/v) as a preservative. Stable for > 2 years at 4°C. Bottle 4: NADP+ plus ATP. Stable for > 2 years at 4°C. Bottle 5: Hexokinase plus glucose-6-phosphate dehydrogenase suspension, 2.2 mL. Stable for > 2 years at 4°C. Bottle 6: 6-Phosphogluconate dehydrogenase suspension (2.2 mL). Stable for > 2 years at 4°C. Bottle 7: Phosphoglucose isomerase suspension (2.2 mL). Stable for > 2 years at 4°C. Bottle 8: Lactulose (0.1 mg/mL) plus D-fructose (0.05 mg/mL) standard solution (5 mL). Stable for > 2 years at 4°C. 温馨提示:需要详细的产品使用说明书,请联系甄准生物客服人员,我们将竭诚为您服务。 甄准,甄心倾听您每一个标准! |