α-葡萄糖醛酸酶检测试剂盒 英文名称: α-D-Glucuronidase assay Kit |
型号:null 产品货号: K-AGLUA |
价格:请致电:010-57128832,18610462672 |
品牌: 爱尔兰 |
品牌: Megazyme 产品货号:K-AGLUA 品牌:Megazyme 产品品名:α-葡萄糖醛酸酶检测试剂盒 英文品名:α-D-Glucuronidase assay Kit 规格型号:50 Assays per kit A simple, reliable and accurate method for the measurement ofalpha-D-glucuronidase in various enzyme preparations. Containing andaldouronic acid substrate and an α-D-glucuronidase control. INTRODUCTION: After cellulose, xylan is the second most abundant polysaccharide found in nature. Xylan is a hemicellulose comprising a β-(1,4)- linked xylan back bone substituted with various side chains such as acetyl groups, α-(1-2/3)-linked L-arabinofuranose and α-(1-2)-linked 4-O-methylglucuronic acid. A repertoire of enzymes are required to effect the complete degradation of plant cell wall polysaccharides in the utilisation of plant biomass. Hence, complete degradation of xylan requires the concerted action of various enzymes including endo-β- (1,4)-xylanases, β-(1,4)-xylosidases, acetylxylan esterases, α-L-arabinofuranosidases and α-glucuronidases. The importance of carbohydrate degrading enzymes has increased over recent years due to the growing focus of obtaining renewable energy from plant biomass in the form of biofuels such as cellulosic and hemicellulosic ethanol. Various research programmes are striving to develop optimised enzyme mixtures or modified microorganisms to increase the efficiency of biomass conversion and, as such, it is important that the various enzyme activities can be easily measured. Simple methods of measuring enzyme activity for some of the well studied carbohydrate degrading enzymes such as endo-β-(1,4)- cellulases, endo-β-(1,4)-xylanases, β-(1,4)-xylosidases exist. However for some enzymes such as α-glucuronidases, current available methods are cumbersome. This kit (K-AGLUA) provides a simple method suitable for the specific measurement of α-D-glucuronidase activity (α-D-glucosiduronate glucuronohydrolase; EC 3.2.1.139 and xylan α-1,2-glucuronosidase; EC 3.2.1.131). KITS: Kits suitable for performing 50 assays in manual format (or 200 assays in microplate format) are available from Megazyme. The kits contain the full assay method plus: Bottle 1: Aldouronic acid mixture (tri:tetra:penta) (6 mL; 10 mg/mL). Stable for > 5 years at -20°C. Bottle 2: α-D-Glucuronidase solution (2 mL; ~ 1.0 U/mL, see label on bottle). Stable for > 2 years at -20°C. Bottle 3: Stop Buffer (28 mL, pH 10) plus sodium azide (0.02 % w/v) as a preservative. Stable for > 2 years at 4°C. Bottle 4: NAD+ freeze dried powder. Stable for > 5 years at -20°C. Bottle 5: Uronate dehydrogenase suspension (1.1 mL). Stable for > 2 years at 4°C. 温馨提示:需要详细的产品使用说明书,请联系甄准生物客服人员,我们将竭诚为您服务。 甄准,甄心倾听您每一个标准! |