LDH细胞毒性检测试剂盒 英文名称: LDH | ||||||||||||||||||||||||||||||||||||||||
型号:null 产品货号: 8078 | ||||||||||||||||||||||||||||||||||||||||
价格:请致电:010-57128832,18610462672 | ||||||||||||||||||||||||||||||||||||||||
品牌: 美国sciencell | ||||||||||||||||||||||||||||||||||||||||
北京裕恒丰科技有限公司,已成功代理美国Sciencell公司细胞生物学产品十余年。多年来,裕恒丰一直秉承信誉至上的原则,在点滴中慢慢地提升企业信誉,树立企业形象,我们始终以热心的服务和专业的技术支持,为广大科研工作者提供了优质的细胞及分子生物学产品。在未来的发展中,我们将继续努力打造企业品牌,北京裕恒丰不仅希望您能聆听到我们的心声,更期待您能亲自体验我们的服务,北京裕恒丰欢迎您的光临。 温馨提示:ScienCell产品仅用于科研,不能用于人或动物以及体外诊断应用。如预购买产品请登陆ScienCell唯一官网(www.sciencellonline.com)寻找正规代理商,以确保您的实验顺利进行。 LDH细胞毒性检测试剂盒 货号8078 Lactate dehydrogenase (LDH), which is a soluble cytosolic enzyme present in most eukaryotic cells, releases into culture medium upon cell death due to damage of plasma membrane. The increase of the LDH activity in culture supernatant is proportional to the number of lysed cells. ScienCell LDH Cytotoxicity Assay kit provides a colorimetric method to measure LDH activity using a reaction cocktails containing lactate, NAD+, diaphrose and INT. LDH catalyses the reduction of NAD+ to NADH in the presence of L-lactate, while the formation of NADH can be measured in a coupled reaction in which the tetrazolium salt INT is reduced to a red formazan product. The amount of the highly colored and soluble formazan can be measured at 490 nm spectrophotometrically.
|