- 偶联物HRP
- 经测试应用IHC-P, WBmore details
- 种属反应性
与反应: Human 预测可用于: Mouse, Rat  - 免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Tyrosine Hydroxylase. A synthetic peptide corresponding to residues surrounding serine 70 of human Tyrosine Hydroxylase. - 阳性对照
- WB: Human adrenal gland lysate. IHC: Human cerebellum tissue.
- 常规说明
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487. Alternative versions available: Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875) Anti-Tyrosine Hydroxylase antibody (Alexa Fluor® 488) [EP1533Y] (ab192463) 性能 - 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
- 存储溶液pH: 7.40
Preservative: 0.1% Proclin Constituents: 30% Glycerol, 1% BSA, PBS -
- 纯度Immunogen affinity purified
- 克隆单克隆
- 克隆编号EP1533Y
- 同种型IgG
- 研究领域
应用Our Abpromise guarantee covers the use of ab193083 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. 应用 | Abreviews | 说明 | IHC-P | | 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ab199507-Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody. | WB | | 1/5000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). | 靶标 - 功能Plays an important role in the physiology of adrenergic neurons.
- 组织特异性Mainly expressed in the brain and adrenal glands.
- 通路Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
- 疾病相关Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. - 序列相似性Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
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Anti-Tyrosine Hydroxylase antibody [EP1533Y] (HRP) 图像 -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (HRP) (ab193083) IHC image of Tyrosine Hydroxylase staining in a section of formalin-fixed paraffin-embedded normal human cerebellum tissue, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab193083 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (HRP) (ab193083) Anti-Tyrosine Hydroxylase antibody [EP1533Y] (HRP) (ab193083) at 1/5000 dilution + Adrenal (Human) Whole Cell Lysate - adult normal tissue (ab29249) at 10 µg developed using the ECL technique Performed under reducing conditions. Predicted band size : 59 kDa Observed band size : 59 kDa Exposure time : 8 seconds This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab193083 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406. |