• 经测试应用WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
• 种属反应性
  与反应: Mouse, Rat, Chicken, Human, Fish, Monkey, Zebrafish, Xenopus tropicalis
• 免疫原

  Recombinant fragment within Mouse GAPDH aa 100 to the C-terminus. The exact sequence is proprietary.
  Database link: P16858

  Run BLAST with Run BLAST with 
• 阳性对照
  • WB: HeLa, UMNSAH/DF-1, Jurkat, COS-1, RAW 264.7 and PC-12 whole cell lysates; Human fetal brain and heart lysates; Xenopus(X. tropicalis) muscle lysate; Zebrafish lysate; Mouse kidney and spleen lysates; Rat brain lysate. IHC-P: Human transitional cell carcinoma of bladder, Mouse spleen and Rat spleen tissues. ICC/IF: HeLa cells. Flow: Jurkat cells. IP: HeLa whole cell extract
• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Alternative versions available:

  Anti-GAPDH antibody (Alexa Fluor^® 488) [EPR16891] (ab201768)
  Anti-GAPDH antibody (Alexa Fluor^® 647) [EPR16891] (ab201272)

  Anti-GAPDH antibody (HRP) [EPR16891] (ab201822)

   

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
• 浓度Batch dependent within range: 100 µl 浓度为 0.925 - 1.114 mg/ml40 µl 浓度为 0.925 mg/ml Batch dependent within range:10 µl 浓度为 0.925 - 1.114 mg/ml 
  

  查找您批次的浓度
• 纯度Protein A purified
• 克隆单克隆
• 克隆编号EPR16891
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • Neurology process
   
  • Neurodegenerative disease
  • Huntington's disease

  • Neuroscience
   
  • Neurology process
   
  • Neurodegenerative disease
  • Alzheimer's disease
   
  • Other

  • Isotype/Loading Controls
   
  • Loading Controls
   
  • GAPDH

  • Signal Transduction
   
  • Metabolism
   
  • Energy Metabolism

  • Cancer
   
  • Cancer Metabolism
   
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Metabolism
   
  • Pathways and Processes
   
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
   
  • Pathways and Processes
   
  • Metabolic signaling pathways
  • Energy transfer pathways
   
  • Energy Metabolism

  • Metabolism
   
  • Types of disease
   
  • Neurodegenerative disease

应用

靶标

• 功能Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
• 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
• 序列相似性Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
• 翻译后修饰S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
  ISGylated.
• 细胞定位Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

• Anti-GAPDH antibody [EPR16891] 图像

  • 

    Western blot - Anti-GAPDH antibody [EPR16891] (ab181602)
    All lanes : Anti-GAPDH antibody [EPR16891] (ab181602) at 1/50000 dilution
    
    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 2 : Xenopus(X. tropicalis) muscle lysates
    Lane 3 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates
    Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
    
    Lysates/proteins at 20 µg per lane.
    
    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
    
    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • 

    Western blot - Anti-GAPDH antibody [EPR16891] (ab181602)
    All lanes : Anti-GAPDH antibody [EPR16891] (ab181602) at 1/10000 dilution
    
    Lane 1 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates
    Lane 2 : Zebrafish lysates
    
    Lysates/proteins at 20 µg per lane.
    
    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
    
    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • 

    Western blot - Anti-GAPDH antibody [EPR16891] (ab181602)
    All lanes : Anti-GAPDH antibody [EPR16891] (ab181602) at 1/10000 dilution
    
    Lane 1 : Human fetal brain lysates
    Lane 2 : Human fetal heart lysates
    
    Lysates/proteins at 10 µg per lane.
    
    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
    
    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • 

    Western blot - Anti-GAPDH antibody [EPR16891] (ab181602)
    All lanes : Anti-GAPDH antibody [EPR16891] (ab181602) at 1/10000 dilution
    
    Lane 1 : Mouse kidney lysates
    Lane 2 : Mouse spleen lysates
    Lane 3 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 5 : Rat brain lysates
    
    Lysates/proteins at 10 µg per lane.
    
    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
    
    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] (ab181602)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling GAPDH with ab181602 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic and nucleus staining on the tumor cells of transitional cell carcinoma of Human bladder is observed. Counter stained with Hematoxylin.

     

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] (ab181602)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling GAPDH with ab181602 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasmic staining on lymphocytes of mouse spleen is observed. Counter stained with Hematoxylin.

     

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] (ab181602)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling GAPDH with ab181602 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasmic staining on lymphocyte of rat spleen is observed. Counter stained with Hematoxylin.

     

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • 

    Immunoprecipitation - Anti-GAPDH antibody [EPR16891] (ab181602)

    GAPDH was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab181602 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab181602 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract. Lane 2: PBS instead of HeLa whole cell extract.

    
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • 

    Flow Cytometry - Anti-GAPDH antibody [EPR16891] (ab181602)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling GAPDH with ab181602 at 1/180 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.