Background: GNAQ is a protein that in humans encoded by the GNAQ gene. The GNAQ protein, an alpha subunit in the Gq class, couples a seven-transmembrane domain receptor to activation of phospholipase C-beta. Mutations at this locus have been associated with problems in platelet activation and aggregation.
Immunogen: A synthetic peptide from the internal region of GNAQ which includes the mutation of Q209P, human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes Q209P mutant, but not wild-type GNAQ of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Western blot:
Western blot analysis of recombinant GNAQ (Q209P) and wildtype proteins. Purified His-tagged GNAQ (Q209P) protein (lane2) and corresponding wild-type protein (lane1) were blotted with anti-GNAQ (Q209P) monoclonal antibody (Cat. #26329).
Immunofluorescence::
Immunofluorescence of cells expressing GNAQ proteins with anti-GNAQ (Q209P) antibody. HEK293T cells were transfected with pCDNA3-GFP-GNAQ (Q209P) plasmid , pCDNA3-GFP-GNAQ (WT) plasmid, pCDNA3-GFP-GNAQ (Q209L) plasmid or pCDNA3-GFP-GNAQ (Q209R) plasmid, then fixed and stained with anti-GNAQ (Q209P) monoclonal antibody (Cat. #26329).