Anti-Gαi Rabbit Polyclonal Antibody
英文名称: Anti-Gαi Rabbit Polyclonal Antibody
型号:null    抗体货号: 21006
价格:请致电:010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

Gαi [21006]
[100 µL]
 

Catalog Number:  21006
Gene Symbol:  i
Description:   Anti-Gα i Rabbit Polyclonal Antibody
Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gα i represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gα i proteins are being explored.
Immunogen:   Recombinant full length protein of Gα i1
Applications:   ELISA, WB, IHC
Recommended Dilutions:   ELISA 1:100-1:5000, WB 1:100-1:2000
Concentration:   1 mg/ml
Host:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   
Purity:   Purified from serum
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  i antibody recognizes Gα i1, Gα i2, and Gα i3 of vertebrates
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of Gi proteins. Gα i1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blot with the polyclonal anti- Gα i antibody (cat # 21006).
 

 

Publications:
1.  Identification of novel signalling roles and targets for Galpha and Gbetagamma downstream of the insulin-like growth factor 1 receptor in vascular smooth muscle cells
    Biochem J. 2013 Feb 15;450(1):209-19
2.  CRH activation of different signaling pathways results in differential calcium signaling in human pregnant myometrium before and during labor
    J Clin Endocrinol Metab. 2012 Oct;97(10):E1851-61
3.  CXCR4 activation defines a new subgroup of Sonic hedgehog-driven medulloblastoma
    Cancer Res. 2012 Jan 1;72(1):122-32
4.  Regulation of estradiol and progesterone production by CRH-R1 and -R2 is through divergent signaling pathways in cultured human placental trophoblasts
    Endocrinology. 2012 Oct;153(10):4918-28
5.  WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins
    Cell Signal. 2011 Mar;23(3):550-4
6.  Heterotrimeric Galpha(i) proteins are regulated by lipopolysaccharide and are anti-inflammatory in endotoxemia and polymicrobial sepsis
    Biochim Biophys Acta. 2011 Mar;1813(3):466-72
7.  Estrogen- and xenoestrogen-induced ERK signaling in pituitary tumor cells involves estrogen receptor-α interactions with G protein-αi and caveolin I
    Steroids Volume 77, Issue 5, April 2012, Pages 424–432
8.  Na/H exchanger regulatory factors control parathyroid hormone receptor signaling by facilitating differential activation of G(alpha) protein subunits
    J Biol Chem. 2010 Aug 27;285(35):26976-86
9.  Beta-arrestin- but not G protein-mediated signaling by the "decoy" receptor CXCR7
    Proc Natl Acad Sci U S A. 2010 Jan 12;107(2):628-32
10.  Structural basis for activation of trimeric Gi proteins by multiple growth factor receptors via GIV/Girdin
    Mol Biol Cell. 2014 Nov 5;25(22):3654-71
11.  Canonical and Non-canonical G-protein Signaling Helps Coordinate Actin Dynamics to Promote Macrophage Phagocytosis of Zymosan
    Mol Cell Biol. 2014 Nov 15;34(22):4186-99
12.  Fatty Acid-binding Protein 5 (FABP5) Regulates Cognitive Function Both by Decreasing Anandamide Levels and by Activating the Nuclear Receptor Peroxisome Proliferator-activated Receptor β/δ (PPARβ/δ) in the Brain
J Biol Chem. 2014 May 2;289(18):12748-58