Anti-MEK1 (D67N) Mouse Monoclonal Antibody
英文名称： Anti-MEK1 (D67N) Mouse Monoclonal Antibody
型号:null    抗体货号： 26224
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

MEK1 (D67N) [26224]
[100ul]		  

Catalog Number:  26224
Gene Symbol:  MAP2K1; MAPKK1; MEK1; MKK1; PRKMK1
Description:   Anti-MEK1 (D67N) Mouse Monoclonal Antibody
Background:   MEK1 (also known as extracellular signal-regulated kinases, ERKs) plays important role in the MAP kinase cascade, which transduces multiple extracellular signals to control cell growth, proliferation and differentiation. Activated upon growth factors stimulation, MEK1 phosphorylates MAPK3/ERK1 and MAPK1/ERK2, thus activates the MAP pathway and regulates transcription. Abnormal of the MEK1 protein, including point mutations, are implicated in diseases such as cardiofaciocutaneous syndrome (CFC) and melanoma.
Immunogen:   A synthetic peptide from the internal region of MEK1 which includes the mutation of D67N, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:2000 
WB: 1:500-1:1000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes D67N mutant, but not wild-type MEK1 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant MEK1 (D67N) and wildtype proteins. Purified His-tagged MEK1 (D67N) protein (lane 1) and corresponding wildtype protein (lane 2) were blotted with anti-MEK1 (D67N) monoclonal antibody (Cat. #26224).
Immunofluorescence:  
Immunofluorescence of cells expressing MEK1 proteins with anti-MEK1 (D67N) antibody. HEK293T cells were transfected with pCDNA3-GFP-MEK1 (WT) plasmid (left column) or pCDNA3-GFP-MEK1 (D67N) plasmid (right column), then fixed and stained with anti-MEK1 (D67N) monoclonal antibody (Cat. #26224).