Anti-EGFR Rabbit Polyclonal Antibody
英文名称：  Anti-EGFR Rabbit Polyclonal Antibody
型号:null    抗体货号： 21272
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

EGFR [21272]
[100 µL]		  

Catalog Number:  21272
Gene Symbol:   EGFR
Description:   Anti-EGFR Rabbit Polyclonal Antibody
Background:   EGFR is a receptor tyrosine kinase for ligands of the EGF family. EGFR activates several signaling cascades to convert extracellular cues into appropriate cellular responses. Defects in EGFR are associated with cancers such as lung cancer.
Immunogen:   Purified EGFR protein, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:100-1:1000 
IHC: 1:50-1:200
Concentration:    0.2mg/ml
Host:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   
Purity:   Purified from serum
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes EGFR of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant EGFR protein. Purified His-tagged EGFR protein was blotted with anti-EGFR rabbit polyclonal antibody (Cat. #21272).
Immunohistochemistry:  
Immunohistochemical analysis of paraffin-embedded human thyroid cancer (upper) and lung cancer (lower) tissue with anti-EGFR polyclonal antibody (Cat. #21272). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37°C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.
Publications:
1.  Immunohistochemistry with a novel mutation-specific monoclonal antibody as a screening tool for the EGFR L858R mutational status in primary lung adenocarcinoma
    Tumor Biology. October 2014