Anti-GNAQ (Q209L) Mouse Monoclonal Antibody
英文名称: Anti-GNAQ (Q209L) Mouse Monoclonal Antibody
型号:null    抗体货号: 26328
价格:请致电:010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

GNAQ (Q209L) [26328]
[100ul]
 

Catalog Number:  26328
Gene Symbol:  GNAQ, CMC1, G-ALPHA-q, GAQ, SWS
Description:   Anti-GNAQ (Q209L) Mouse Monoclonal Antibody
Background:   GNAQ is a protein that in humans encoded by the GNAQ gene. The GNAQ protein, an alpha subunit in the Gq class, couples a seven-transmembrane domain receptor to activation of phospholipase C-beta. Mutations at this locus have been associated with problems in platelet activation and aggregation.
Immunogen:   A synthetic peptide from the internal region of GNAQ which includes the mutation of Q209L, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes Q209L mutant, but not wild-type GNAQ of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant GNAQ (Q209L) and wildtype proteins. Purified His-tagged GNAQ (Q209L) protein (lane2) and corresponding wild-type protein (lane1) were blotted with anti-GNAQ (Q209L) monoclonal antibody (Cat. #26328).
Immunofluorescence:  
Immunofluorescence of cells expressing GNAQ proteins with anti-GNAQ (Q209L) antibody. HEK293T cells were transfected with pCDNA3-GFP-GNAQ (Q209L) plasmid pCDNA3-GFP-GNAQ (WT) plasmid, pCDNA3-GFP-GNAQ (Q209P) plasmid or pCDNA3-GFP-GNAQ (Q209R) plasmid, then fixed and stained with anti-GNAQ (Q209L) monoclonal antibody (Cat. #26328).