| Catalog Number: 28004 | | Gene Symbol: His-tag | | Description: Anti-His-tag Mouse Monoclonal Antibody | | Background: Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. It provides a means of specifically purifying or detecting the recombinant protein without a protein-specific antibody or probe. | | Immunogen: His-tag | | Applications: WB | Recommended Dilutions:
WB 1:1000-1:5000 | | Concentration: 1 mg/ml | | Host: Mouse | | Format: Liquid | | Clonality: Monoclonal | | Isotype: | | Purity: Purified from ascites | | Storage buffer: | | Preservative: no | | Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | | Species Reactivity: His-tag antibody recognizes expressed His-tagged proteins from vertebrates. | | Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles | | | | Western blot: |  |  | | Western blot analysis of recombinant His-tagged proteins. Purified His-tagged Vimentin (Cat. #10190, lane 1), RheB (Cat. #10118, lane 2) and Cystatin C (Cat. #10157, lane 3) proteins were blotted with anti-His-tag mouse monoclonal antibody(Cat.# 28004) at 1:1000 dilution(Top panel). Proteins loading were blotted with anti-Vimentin monoclonal antibody (Cat. #26054), anti-RheB monoclonal antibody (Cat. #26015) and anti-Cystatin C monoclonal antibody(Cat. #26044) at 1:1000 dilution, repectivly(Bottom panel). | | |