美国Brand,null 产品货号： SL100489-MDCKItem#, 预优化DNA转染试剂-MDCK<br>英文名称： GenJet™ In Vitro DNA Tranfection Reagent for MDCK Cells

Description

GenJet™ DNA In Vitro Tranfection Reagent for MDCK cell is pre-optimized for transfecting MDCK cells. MDCK cell line is derived from the kidney of an apparently normal, adult, female cocker spaniel; used in numerous viral studies; susceptible to vesicular stomatitis, vaccinia, and Coxsackie viruses, and certain reoviruses and adenoviruses.

The "Shaved Cell Transfection" protocol was optimized to achieve maximal transfection efficiency. Refer to the following optimal transfection conditions for maximal transfection efficiency on MDCK cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 transfections in 24 well plates or 150 transfections in 6 well plates.

Summary of Optimal Shaved CellTransfection Conditions:
Cell culture medium
Confluency of cell on the day of shaving
Cell density for plating
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Incubation time of shaved cells with transfection complex
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)

DMEM with 4.5 g/L glucose, 10% FBS
95~100%
1.3x10^5 cells per square centimeter
4:1
Serum-free DMEM with 4.5 g/L glucose
12 minutes at RT
20 minutes at 37 degree
48 hours

EGFP
pEGFP-N3 (CMV promoter)
70%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

A Picture Showing Transfection Efficiency of GenJet™ Reagent on MDCK Cells. MDCK cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection

Data Sheet.pdf