Lonza Nucleofector 细胞核转染试剂盒 VVPI-1006
英文名称： 100 RCT Basic Glial Cells Kit
型号:null    产品货号： VVPI-1006
价格:请致电：010-57128832,18610462672
品牌: 德国进口

北京泽平代理的Lonza Nucleofector 细胞核转染仪 (原名Amaxa Nucleofector)，通过电穿孔技术，配合专用电转染试剂盒，能将DNA、RNA等物质导入细胞，并直接穿过核膜进入细胞核，实现高效转染。北京泽平是Lonza中国区一级代理，销售Lonza旗下全系列产品，如Lonza Nucleofector细胞核转染系统、CytoSMART活细胞成像系统、原代细胞和培养基、基础细胞培养基（DMEM、MEM、RPMI 1640）、内毒素检测试剂盒、支原体检测试剂盒及清除试剂盒、琼脂糖和闪胶等。自2009年起北京泽平成为Lonza全国代理，凭借优异的产品和服务，受到广大客户的认可！欢迎来电咨询北京泽平官方客服400-686-0100或13910005869或13521452266

conditions for maximal transfection efficiency on Jurkat cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency 

Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)


~70%
PRMI with 10% FBS
3:1
1xGenJet™ Transfection Buffer
15 minutes at RT
Yes
No
48 hours


EGFP
pEGFP-N3 (CMV promoter) 
80%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet & Protocol.pdf