预优化siRNA转染试剂-Hela
英文名称： GenMute™ siRNA Transfection Reagent for Hela Cell
型号:null    产品货号： SL100568-HELA
价格:请致电：010-57128832,18610462672
品牌: 美国

Description:
GenMute™ siRNA Transfection Reagent for Hela Cell is pre-optimized for transfecting siRNA to Hela cells. GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 10 pmoles siRNA or miRNA mimics in 24-well plate.

Refer to the following optimal transfection conditions for maximal silencing on Hela cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Cell culture medium
Confluency of cell on the day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute™ Reagent
Incubation Time to Form GenMute™/siRNA Complex
Maximal Silencing Efficiency 

Transfection Results:
Reporter Gene
Silencing Efficiency (% )


DMEM with 4.5 g/L glucose, 10% FBS
~50%
20.0 nM
1xGenMute™ Transfection Buffer
~15 minutes at RT
48 hours


Renilla Luciferase
90%


Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Data Sheet & Protocol.pdf

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储存：-70°C储存至少6个月转化效率不变

 

HIT 感受态细胞优势：只需使用冰块！
与传统感受态细胞相比：

l  无需热刺激

l  10min以内完成转化步骤

l  真正1步/1管过程

l  保证效率高达 108 cfu/ug 质粒

l  增加冻融耐性

l  简单的操作过程适合于自动化

 

HIT感受态细胞操作步骤：

 

新一代感受态细胞，无需热刺激！
只需10mins简单操作，高效转化！
HIT competent cell protocol
 

 

(1-10 minutes, efficiency=108 cfu/µg)

注意：转化前请准备好冰浴，将平板培养基与玻璃棒干燥预热到37℃

 

1) 从-70℃冰箱取出感受态细胞置于冰浴中10~20秒，至解冻1/3。
 
2) 加入待转化的DNA(其中DNA 体积不应超过感受态细胞体积的1/10)。漩涡振荡离心管1秒，混匀内容物。
 
3) 控制冰浴中静置1-10分钟（建议可以静置5分钟）。
 
4) 转移至37℃干燥平板培养基(含相应抗生素的SOC或LB固体琼脂培养基等)上, 用玻璃棒涂板。
   平板置于 37℃培养16-18 小时，观察转化克隆的增长 
参考文献

a.Identification and characterization of a novel enzyme related to the synthesis of PUFAs derived from Thraustochytrium aureum ATCC 34304

DH Kang, P Anbu, YS Jeong, BP Chaulagain… - Biotechnology and …, 2010 - Springer

b.         Phenotypic and genetic characterization of Erwinia rhapontici isolated from diseased Asian pear fruit trees

SP Thapa, SY Cho, JH Hur, CK Lim - Phytoparasitica, 2012 - Springer

c.         Development of SCAR marker for simultaneous identification of Miscanthus sacchariflorus, M. sinensis and M. x giganteus

JK Kim, GH An, SH Ahn, YH Moon, YL Cha… - Bioprocess and …, 2012 - Springer

d.         Enhanced 2, 3-Butanediol Production in Recombinant Klebsiella pneumoniae via Overexpression of Synthesis-Related Genes

KB Rim, JH Park, MK Oh, JW Lee - Journal of Microbiology and …, 2012 - papersearch.net